Department of Oral Biology, Faculty of Dentistry, Ain Shams University, Cairo, Egypt.
Department of Oral Biology, Faculty of Dentistry, Ain Shams University, Cairo, Egypt.
Acta Histochem. 2019 Oct;121(7):812-822. doi: 10.1016/j.acthis.2019.07.007. Epub 2019 Jul 26.
Diabetes mellitus (DM) is a chronic metabolic disease characterized by high blood glucose levels. DM affects many body's organs and caused by insulin production deficiency or by the ineffectiveness of the produced insulin. Administration of exogenous insulin is required for management of type I DM; however, it does not cure the disease. Bone marrow-mesenchymal stem cells (BM-MSCs) have been highlighted to offer a novel cell based approach for treatment of diabetes because of their anti-diabetic effect, direct differentiation into a variety of cell types, or release of paracrine factors.
To examine the effect of BM-MSCs versus insulin on true filiform and fungiform papillae of diabetic rats.
Fifty six male Wistar albino rats weighing 200-250 g were equally divided into: Control group (Gp I): Rats did not receive any drug. Diabetic group (Gp II): Rats received a single intra-peritoneal injection of streptozotocin (40 mg/kg). BM-MSCs treated diabetic group (Gp III): After DM confirmation; rats received a single intravenous injection of BM-MSCs (million units) through tail vein. Insulin treated diabetic group (Gp IV): After DM confirmation; rats received a daily subcutaneous injection of insulin (5IU/kg). After four weeks, half of the tongue specimens were processed and stained by Hematoxyline & Eosin and Anti-proliferating cell nuclear antigen (Anti-PCNA) then examined by light microscope. Fluorescent microscope was used to detect homing of injected labeled BM-MSCs in rats' filiform and fungiform papillae. While the other half were examined by scanning electron microscope.
True filiform and fungiform papillae of Gp II showed significant histological and morphological alterations. In treated groups, Gp III and Gp IV, both papillae showed marked improvements, being more noticeable in Gp IV. There was a significant increase in the number of Anti-PCNA positive cells and a significant decrease in fasting blood glucose level in Gp III and Gp IV in comparison to Gp II.
DM had degenerative effects on true filiform and fungiform papillae. Administration of BM-MSCs reduced the deleterious effects of DM on both papillae. Insulin injection caused more obvious improvements in both papillae of diabetic rats than BM-MSCs.
糖尿病（DM）是一种以高血糖为特征的慢性代谢性疾病。DM 影响许多身体器官，是由胰岛素产生不足或产生的胰岛素无效引起的。I 型 DM 的管理需要外源性胰岛素；然而，它并不能治愈这种疾病。骨髓间充质干细胞（BM-MSCs）因其抗糖尿病作用、直接分化为多种细胞类型或释放旁分泌因子而被突出为治疗糖尿病的一种新的基于细胞的方法。
研究 BM-MSCs 与胰岛素对糖尿病大鼠真性丝状和菌状乳头的影响。
56 只雄性 Wistar 白化大鼠，体重 200-250g，平均分为 4 组：对照组（Gp I）：大鼠未接受任何药物。糖尿病组（Gp II）：大鼠腹腔注射链脲佐菌素（40mg/kg）。BM-MSCs 治疗糖尿病组（Gp III）：确认 DM 后；大鼠尾静脉注射单次静脉注射 BM-MSCs（百万单位）。胰岛素治疗糖尿病组（Gp IV）：确认 DM 后；大鼠每天皮下注射胰岛素（5IU/kg）。四周后，一半的舌标本用苏木精和伊红染色，并用抗增殖细胞核抗原（Anti-PCNA）染色，然后在光镜下检查。荧光显微镜用于检测注射标记的 BM-MSCs 在大鼠丝状和菌状乳头上的归巢。另一半用扫描电子显微镜检查。
Gp II 的真性丝状和菌状乳头表现出明显的组织学和形态学改变。在治疗组 Gp III 和 Gp IV 中，两种乳头均有明显改善，Gp IV 更为明显。与 Gp II 相比，Gp III 和 Gp IV 的 Anti-PCNA 阳性细胞数量显著增加，空腹血糖水平显著降低。
DM 对真性丝状和菌状乳头有退行性影响。BM-MSCs 的给药减少了 DM 对两种乳头的有害影响。与 BM-MSCs 相比，胰岛素注射对糖尿病大鼠的两种乳头都有更明显的改善。
