脑啡肽转换酶：使用[3H]胍基乙硫基琥珀酸进行的特性鉴定与定位

Enkephalin convertase: characterization and localization using [3H]guanidinoethylmercaptosuccinic acid.

作者信息

Lynch D R, Venable J C, Strittmatter S M, Snyder S H

机构信息

Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205.

出版信息

Biochimie. 1988 Jan;70(1):57-64. doi: 10.1016/0300-9084(88)90158-7.

DOI:10.1016/0300-9084(88)90158-7

PMID:3135843

Abstract

Enkephalin convertase (carboxypeptidase E,H; EC 3.4.17.10) is a carboxypeptidase B-like enzyme which appears to be physiologically associated with the biosynthesis of the enkephalins and certain other peptides. We have localized enkephalin convertase in the brain and other tissues autoradiographically by labeling studies with [3H]guanidinoethylmercaptosuccinic acid ([3H]GEMSA). In the brain, [3H]GEMSA localizations parallel enkephalin distribution but with certain exceptions, suggesting a role in relation to other peptides. In the pancreas, [3H]GEMSA binding sites are localized to the islets suggesting an involvement in insulin, glucagon, or somatostatin formation. The selective concentration of [3H]GEMSA grains in cardiac atria suggests a link to atrial natriuretic factor.

摘要

脑啡肽转换酶（羧肽酶E，H；EC 3.4.17.10）是一种类羧肽酶B的酶，似乎在生理上与脑啡肽及某些其他肽的生物合成相关。我们通过用[3H]胍基乙硫基琥珀酸（[3H]GEMSA）进行标记研究，以放射自显影法在脑和其他组织中定位了脑啡肽转换酶。在脑中，[3H]GEMSA的定位与脑啡肽分布平行，但有某些例外，提示其与其他肽有关。在胰腺中，[3H]GEMSA结合位点定位于胰岛，提示其参与胰岛素、胰高血糖素或生长抑素的形成。[3H]GEMSA颗粒在心房中的选择性聚集提示其与心钠素有关。