Gu Yu, Zhao Bo-Wen, Chen Xi, Luo Yin-Zhen, Zhang Yan-Ling
State Laboratory of Traditional Chinese Medicine Information Engineering, State Administration of Traditional Chinese Medicine,School of Chinese Material Medica, Beijing University of Chinese Medicine Beijing 100102, China.
Zhongguo Zhong Yao Za Zhi. 2019 Jun;44(11):2353-2358. doi: 10.19540/j.cnki.cjcmm.20190111.006.
In this paper, Xixian Tongshuan Preparation was used as the research object, and all the chemical components of the 13 traditional Chinese medicines were collected. The target finding technique was used to obtain the key targets of the neuroprotective effect of Xixian Tongshuan Preparation, including 5 glutamate receptors, TGFR-1 and VEGFR-2. Molecular docking technology was used to screen out the potential active components of the above targets and to analyze their mechanism of action. It was found that single component, such as neo-complanatoside and neo-carthamin, in Xixian Tongshuan Preparation could simultaneously act on different targets. The chemical constituents in Ligusticum chuanxiong, Angelica sinensis, Carthamus tinctorius, and Panax pseudo-ginseng could simultaneously act on different neuroprotective-related targets, which reflected the application of multi-components to multi-targets. Point and multiple sites played a key role in protecting neurons against cerebral ischemic injury. This study explains the multi-target mechanism of anti-cerebral ischemic injury in neuroprotection at the molecular level, and provides a certain direction for the clinical application and experimental research of Xixian Tongshuan Preparation.
本文以熄痫通栓制剂为研究对象，收集了13味中药的所有化学成分。采用靶点发现技术获得熄痫通栓制剂神经保护作用的关键靶点，包括5种谷氨酸受体、TGFR - 1和VEGFR - 2。运用分子对接技术筛选上述靶点的潜在活性成分并分析其作用机制。研究发现，熄痫通栓制剂中的新异土木香苷、新红花苷等单一成分可同时作用于不同靶点。川芎、当归、红花和三七中的化学成分可同时作用于不同的神经保护相关靶点，体现了多成分作用于多靶点。靶点和多位点在保护神经元免受脑缺血损伤中起关键作用。本研究从分子水平阐释了熄痫通栓制剂抗脑缺血损伤的多靶点机制，为熄痫通栓制剂的临床应用和实验研究提供了一定方向。
