Zhang Yin-Huan, Yang Xiao-Wei, Dai Yi-Hang, Xiao Hong-Bin
School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 100029,China.
Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
Zhongguo Zhong Yao Za Zhi. 2019 Jul;44(13):2820-2826. doi: 10.19540/j.cnki.cjcmm.20190321.401.
The aim of this study was to explore the effect of emodin on lipid accumulation and inflammation in hepatocytes. The cell morphology was observed by microscopy. LDH release was detected by the kit. Levels of intracellular lipid droplets were observed by oil red O staining. The contents of TC and TG in cells were detected by the kit. Western blot was used to determine protein expressions of FASN,SREBF2,APOB,IL-6 and p-NF-κB in hepatocytes. The results showed that the levels of L02 cell LDH were significantly increased after being treated with emodin,and the cells showed shrinkage,volume reduction,decrease in quantity with the increase of dose. Red lipid droplets were observed in L02 hepatocytes. Intracellular TC and TG contents of L02 cell increased in a concentrationdependent manner,with significant differences between medium and high-dose groups( P < 0. 05). Protein expressions of FASN,SREBF2,IL-6 and p-NF-κB were significantly higher than those of the control group,and the expression level of APOB was significantly lower than that of the control group( P<0. 05). In conclusion,emodin could induce lipid accumulation and inflammatory damage in hepatocytes in a dose-dependent manner,which in turn could damage liver cells. This process was related to the up-regulation of FASN,SREBF2,IL-6,p-NF-κB,as well as the down-regulation of the protein expression of APOB.
本研究旨在探讨大黄素对肝细胞脂质蓄积和炎症的影响。通过显微镜观察细胞形态。使用试剂盒检测乳酸脱氢酶（LDH）释放。通过油红O染色观察细胞内脂滴水平。使用试剂盒检测细胞中总胆固醇（TC）和甘油三酯（TG）的含量。采用蛋白质免疫印迹法检测肝细胞中脂肪酸合酶（FASN）、固醇调节元件结合蛋白2（SREBF2）、载脂蛋白B（APOB）、白细胞介素6（IL-6）和磷酸化核因子κB（p-NF-κB）的蛋白表达。结果显示，大黄素处理后L02细胞LDH水平显著升高，且细胞出现皱缩、体积减小、数量随剂量增加而减少。在L02肝细胞中观察到红色脂滴。L02细胞内TC和TG含量呈浓度依赖性增加，中、高剂量组之间差异有统计学意义（P<0.05）。FASN、SREBF2、IL-6和p-NF-κB的蛋白表达显著高于对照组，APOB的表达水平显著低于对照组（P<0.05）。综上所述，大黄素可呈剂量依赖性诱导肝细胞脂质蓄积和炎性损伤，进而损伤肝细胞。这一过程与FASN、SREBF2、IL-6、p-NF-κB的上调以及APOB蛋白表达的下调有关。
