Nerudová J, Gut I, Savolainen H
Institute of Hygiene and Epidemiology, Prague, Czechoslovakia.
Environ Res. 1988 Aug;46(2):133-41. doi: 10.1016/s0013-9351(88)80027-6.
Acrylonitrile caused thiobarbituric acid-positive reactants time- and concentration-dependently in isolated hepatocytes. This effect was markedly enhanced by gassing of the medium with 95% oxygen-5% CO2 gas mixture. Glycidonitrile, an acrylonitrile metabolite, proved more potent in this respect than the parent acrylonitrile or its end metabolite, cyanide anion. The latter decreased greatly the viability of isolated liver cells but caused thiobarbituric acid-positive reactants only in the presence of diethylmaleate. Acrylonitrile caused also a decrease in the concentration of nonprotein sulfhydryl groups but the oxidation of glutathione (GSH) to GSSG (oxidized glutathione) was not the major mechanism. This might indicate the consumption of GSH in the glutathione S-transferase catalyzed reactions. In contrast to cyanide anion-induced effects acrylonitrile did not affect markedly the viability of hepatocytes.
丙烯腈在分离的肝细胞中呈时间和浓度依赖性地引起硫代巴比妥酸阳性反应物。用95%氧气-5%二氧化碳气体混合物对培养基进行通气可显著增强这种效应。环氧丙腈是丙烯腈的一种代谢产物，在这方面比母体丙烯腈或其终代谢产物氰根阴离子更具效力。后者大大降低了分离的肝细胞的活力，但仅在顺丁烯二酸二乙酯存在的情况下才会引起硫代巴比妥酸阳性反应物。丙烯腈还会导致非蛋白巯基浓度降低，但谷胱甘肽（GSH）氧化为氧化型谷胱甘肽（GSSG）并非主要机制。这可能表明谷胱甘肽在谷胱甘肽S-转移酶催化的反应中被消耗。与氰根阴离子诱导的效应相反，丙烯腈对肝细胞的活力没有明显影响。
