SKF 525A对内皮细胞释放一氧化氮和前列环素的影响。

Effect of SKF 525A on the release of nitric oxide and prostacyclin from endothelial cells.

作者信息

Rees D D, Palmer R M, Moncada S

机构信息

Wellcome Research Laboratories, Beckenham, Kent, U.K.

出版信息

Eur J Pharmacol. 1988 May 20;150(1-2):149-54. doi: 10.1016/0014-2999(88)90761-3.

DOI:10.1016/0014-2999(88)90761-3

PMID:3136027

Abstract

The effect of SKF 525A on the endothelium-dependent relaxation of rabbit aortic rings and on the release of nitric oxide and prostacyclin from porcine aortic endothelial cells in culture was examined. SKF 525A (10-30 microM) inhibited acetylcholine- but not the calcium ionophore A23187-induced endothelium-dependent relaxation without affecting the release of endothelium-derived relaxing factor from endothelial cells stimulated with bradykinin. Higher concentrations of SKF 525A (30-200 microM) caused transient endothelium-dependent relaxation of rabbit aortic rings and the release of nitric oxide and prostacyclin from the endothelial cells without inducing release of lactate dehydrogenase. The mechanism whereby SKF 525A releases nitric oxide and prostacyclin from endothelial cells requires further investigation.

摘要

研究了SKF 525A对兔主动脉环内皮依赖性舒张以及对培养的猪主动脉内皮细胞中一氧化氮和前列环素释放的影响。SKF 525A（10 - 30微摩尔）抑制乙酰胆碱诱导的内皮依赖性舒张，但不抑制钙离子载体A23187诱导的内皮依赖性舒张，且不影响缓激肽刺激的内皮细胞释放内皮源性舒张因子。更高浓度的SKF 525A（30 - 200微摩尔）引起兔主动脉环短暂的内皮依赖性舒张以及内皮细胞释放一氧化氮和前列环素，且不诱导乳酸脱氢酶的释放。SKF 525A从内皮细胞释放一氧化氮和前列环素的机制需要进一步研究。