Yoshida M A, Ikeuchi T, Tachibana Y, Takagi K, Moriyama M, Tonomura A
Department of Cytogenetics, Medical Research Institute, Tokyo.
Jpn J Cancer Res. 1988 May;79(5):600-7. doi: 10.1111/j.1349-7006.1988.tb00029.x.
Cytogenetic studies were successfully carried out in 5 tumor tissues from Japanese patients with nonfamilial renal cell carcinoma, histologically diagnosed as clear cell subtype. Mitotic cells were obtained by a combined method of enzymatic disaggregation and short-term culture (6-12 days). The modal chromosome numbers were found to be diploid or near-diploid in all the cases examined. Every case showed characteristic structural and numerical abnormalities. Rearrangements in the short arm of chromosome 3 were observed as clonal abnormalities in all the cases, including a translocation t(3;6) resulting in a partial loss of 3p (3 cases), a terminal deletion of 3p (one case) and 2 different translocations involving 3p and 8p (one case). The other clonal abnormalities were a whole or partial trisomy of chromosome 7 and a loss of Y chromosome. The overall results in the present study were consistent with those of our previous data in American patients, and suggest that the rearrangements of chromosome 3 leading to a partial loss of its short arm may play primary and significant role(s) in the development of renal cell carcinoma.
对5例来自日本非家族性肾细胞癌患者的肿瘤组织成功进行了细胞遗传学研究，组织学诊断为透明细胞亚型。通过酶解和短期培养（6 - 12天）相结合的方法获得有丝分裂细胞。在所检查的所有病例中，众数染色体数均为二倍体或接近二倍体。每个病例均显示出特征性的结构和数目异常。在所有病例中均观察到3号染色体短臂重排为克隆性异常，包括导致3p部分缺失的易位t(3;6)（3例）、3p末端缺失（1例）以及涉及3p和8p的2种不同易位（1例）。其他克隆性异常为7号染色体的整条或部分三体以及Y染色体缺失。本研究的总体结果与我们之前对美国患者的数据一致，并表明导致3号染色体短臂部分缺失的重排在肾细胞癌的发生中可能起主要和重要作用。
