Department of Rhinology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.
Immunotherapy. 2019 Sep;11(13):1095-1105. doi: 10.2217/imt-2019-0047. Epub 2019 Jul 30.
This study aims to investigate whether microRNA (miR)-146a exerts therapeutic potential to allergic rhinitis (AR). Female BALB/c mice were randomly divided into the AR and miR-146a groups. miR-146a was administered into the nostril before ovalbumin (OVA) challenge daily on days 21-28. The expression level of miR-146a in AR nasal mucosa was significantly decreased. miR-146a mimic markedly attenuated sneezing and nasal rubbing events, decreased the levels of ovalbumin-specific IgE as well as mastocyte- and basophil-related inflammatory cytokines, reduced inflammatory cells, affected the T-helper 2-released cytokines. Furthermore, miR-146a mimic inhibited the expressions of proteins relevant to Toll-like receptor 4 (TLR4)/TRAF6/NF-κB signaling pathway. The anti-inflammatory effects of miR-146a on AR might exert through the inhibition of the toll-like receptor 4 (TLR4)/TRAF6/NF-κB signaling pathway to some extent.
本研究旨在探讨 microRNA（miR）-146a 是否对过敏性鼻炎（AR）具有治疗潜力。 将雌性 BALB/c 小鼠随机分为 AR 组和 miR-146a 组。 在卵清蛋白（OVA）挑战前 21-28 天，每天将 miR-146a 注入鼻腔。 AR 鼻黏膜中 miR-146a 的表达水平显著降低。 miR-146a 模拟物明显减轻打喷嚏和鼻擦次数，降低卵清蛋白特异性 IgE 以及肥大细胞和嗜碱性粒细胞相关炎症细胞因子的水平，减少炎症细胞，影响 Th2 释放的细胞因子。 此外，miR-146a 模拟物抑制与 Toll 样受体 4（TLR4）/ TRAF6 / NF-κB 信号通路相关的蛋白表达。 miR-146a 对 AR 的抗炎作用可能在一定程度上通过抑制 Toll 样受体 4（TLR4）/ TRAF6 / NF-κB 信号通路来发挥作用。
