Institute of Quantitative Biology, Biochemistry and Biotechnology/CSEC, School of Biological Sciences, University of Edinburgh, Edinburgh, EH9 3FF, UK.
EdinOmics, SynthSys, CH Waddington Building, Max Born Crescent, The King's Buildings, Edinburgh, EH9 3BF, UK.
Microbiology (Reading). 2019 Dec;165(12):1282-1294. doi: 10.1099/mic.0.000840.
Platinum and palladium are much sought-after metals of critical global importance in terms of abundance and availability. At the nano-scale these metals are of even higher value due to their catalytic abilities for industrial applications. is able to capture ionic forms of both of these metals, reduce them and synthesize elemental nanoparticles. Despite this ability, very little is known about the biological pathways involved in the formation of these nanoparticles. Proteomic analysis of in response to platinum and palladium has highlighted those proteins involved in both the reductive pathways and the wider stress-response system. A core set of 13 proteins was found in both treatments and consisted of proteins involved in metal transport and reduction. There were also seven proteins that were specific to either platinum or palladium. Overexpression of one of these platinum-specific genes, a NiFe hydrogenase small subunit (Dde_2137), resulted in the formation of larger nanoparticles. This study improves our understanding of the pathways involved in the metal resistance mechanism of and is informative regarding how we can tailor the bacterium for nanoparticle production, enhancing its application as a bioremediation tool and as a way to capture contaminant metals from the environment.
铂和钯是全球范围内稀缺且重要的金属，其丰度和可用性至关重要。在纳米尺度上，由于其在工业应用中的催化能力，这些金属的价值更高。能够捕获这两种金属的离子形式，将其还原并合成元素纳米颗粒。尽管具有这种能力，但对于这些纳米颗粒形成所涉及的生物途径知之甚少。对 响应铂和钯的蛋白质组学分析突出了那些参与还原途径和更广泛应激反应系统的蛋白质。在两种处理中都发现了一组核心的 13 种蛋白质，它们包含参与金属运输和还原的蛋白质。还有 7 种蛋白质是铂或钯特有的。一种铂特异性基因（Dde_2137，镍铁氢化酶小亚基）的过表达导致形成更大的纳米颗粒。这项研究提高了我们对 金属抗性机制中涉及的途径的理解，并为我们如何针对纳米颗粒生产对细菌进行定制提供了信息，增强了其作为生物修复工具的应用，并为从环境中捕获污染物金属提供了一种方法。
