Departamento de Genética, Facultad de Ciencias Biológicas, Universitat de València, Burjassot, Valencia, Spain.
Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia.
J Invertebr Pathol. 2019 Sep;166:107224. doi: 10.1016/j.jip.2019.107224. Epub 2019 Jul 27.
When Colorado potato beetle larvae ingested potato plants treated with the plant defense inducer compound hexanoic acid, midgut chymotrypsin enzyme activity increased, and the corresponding chymotrypsin genes were differentially expressed, evidence of the larval digestive proteolytic system's plasticity. We previously reported increased susceptibility to Cry3Aa toxin in larvae fed hexanoic acid treated plants. Here we show that the most expressed chymotrypsin gene in larvae fed hexanoic acid treated plants, CTR6, was dramatically downregulated in Cry3Aa intoxicated larvae. lde-miR-965-5p and lde-miR-9a-5p microRNAs, predicted to target CTR6, might be involved in regulating the response to hexanoic acid but not to Cry3Aa toxin.
当马铃薯甲虫幼虫摄入用植物防御诱导剂化合物己酸处理过的马铃薯植株时，中肠糜蛋白酶的酶活性增加，相应的糜蛋白酶基因表达差异，这表明幼虫消化蛋白水解系统具有可塑性。我们之前报道过，幼虫在摄入己酸处理过的植物后对 Cry3Aa 毒素的敏感性增加。在这里，我们发现幼虫在摄入己酸处理过的植物后表达量最高的糜蛋白酶基因 CTR6 在 Cry3Aa 中毒的幼虫中显著下调。预测靶向 CTR6 的 ide-miR-965-5p 和 ide-miR-9a-5p microRNAs 可能参与调节对己酸的反应，但不参与调节对 Cry3Aa 毒素的反应。
