State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou 730046, China.
Institute of General and Experimental Biology, Siberian Branch of Russian Academy of Sciences, Sakhyanovoi st. 6, 670047 Ulan-Ude, Russia.
Infect Genet Evol. 2019 Nov;75:103985. doi: 10.1016/j.meegid.2019.103985. Epub 2019 Jul 27.
Infection of Cysticercus tenuicollis, the larval stage of Taenia hydatigena, is extensively found in sheep and pigs and jeopardizes the breeding and meat industry. miRNAs are a subclass of small noncoding regulatory RNAs and closely associated with the pathogenesis and biology of parasites. Here, using HiSeq sequencing we identified 49 known and 2 potential novel miRNAs in C. tenuicollis, of which both thy-miR-71 and -87 were predominant. Using RT-qPCR, 6 selected miRNAs were validated, and thy-miR-71 and -miR-87 were confirmed to be highly expressed, with the copy number of approximately 82,340 ± 2079 and 19,580 ± 609 per 1 ng total RNA, respectively. Similar to other cestodes, T. hydatigena was predicted to have two conserved miRNA clusters thy-miR-71/2c/2b and thy-miR-4989/277, and three members of the former were confirmed to reside sequentially within the genomic region of 253 bp by PCR. The current data provide us a valuable resource for further studies of a role of miRNAs in T. hydatigena biology and infection.
细粒棘球蚴感染是细粒棘球绦虫的幼虫阶段，广泛存在于绵羊和猪中，危害着畜牧业和肉类产业。miRNA 是一类小的非编码调控 RNA，与寄生虫的发病机制和生物学密切相关。在这里，我们使用 HiSeq 测序在细粒棘球蚴中鉴定了 49 个已知和 2 个潜在的新 miRNA，其中 thy-miR-71 和 -87 占主导地位。通过 RT-qPCR 验证了 6 个选定的 miRNA，thy-miR-71 和 -miR-87 的表达水平较高，拷贝数分别约为 1ng 总 RNA 中的 82,340±2079 和 19,580±609。与其他绦虫一样，细粒棘球绦虫被预测有两个保守的 miRNA 簇 thy-miR-71/2c/2b 和 thy-miR-4989/277，并且通过 PCR 证实前簇的三个成员依次位于 253bp 的基因组区域内。目前的数据为进一步研究 miRNA 在细粒棘球绦虫生物学和感染中的作用提供了有价值的资源。
