School of Urban and Environmental Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 44919, Republic of Korea.
Marine Environmental Research Division, National Institute of Fisheries Science (NIFS), Busan, 46083, Republic of Korea.
Environ Monit Assess. 2019 Jul 30;191(8):525. doi: 10.1007/s10661-019-7675-x.
Arsenic contamination in marine environments is a serious issue because some arsenicals are very toxic, increasing the health risks associated with the consumption of marine products. This study describes the development of an improved rapid method for the quantification of arsenic species, including arsenite (As), arsenate (As), arsenocholine (AsC), arsenobetaine (AsB), dimethylarsinic acid (DMA), and monomethyl arsonic acid (MMA), in seaweed, sediment, and seawater samples using high-performance liquid chromatography/inductively coupled plasma-mass spectrometry (HPLC/ICP-MS). ICP-MS based on dynamic reaction cells was used to eliminate spectral interference. Ammonium nitrate- and phosphate-based eluents were used as the mobile phases for HPLC analysis, leading to shorter overall retention time (6 min) and improved peak separation. Arsenicals were extracted with a 1% HNO solution that required no clean-up process and exhibited reasonable sensitivity and peak resolution. The optimized method was verified by applying it to hijiki seaweed certified reference material (CRM, NMIJ 7405-a) and to spiked blank samples of sediment and seawater. The proposed method measured the concentration of As in the CRM as 9.6 ± 0.6 μg/kg dry weight (dw), which is close to the certified concentration (10.1 ± 0.5 μg/kg dw). The recovery of the six arsenicals was 87-113% for the sediment and 99-101% for the seawater. In the analysis of real samples, As was the most abundant arsenical in hijiki and gulfweed, whereas AsB was dominant in other seaweed species. The two inorganic arsenicals (As and As) and As were the most dominant in the sediment and seawater samples, respectively.
海洋环境中的砷污染是一个严重的问题，因为一些砷化物毒性很强，增加了与食用海产品相关的健康风险。本研究描述了一种改进的快速方法的开发，用于定量海洋生物、沉积物和海水中的砷形态，包括亚砷酸盐（As）、砷酸盐（As）、砷胆碱（AsC）、砷甜菜碱（AsB）、二甲基砷酸（DMA）和一甲基胂酸（MMA），使用高效液相色谱/电感耦合等离子体质谱（HPLC/ICP-MS）。基于动态反应池的 ICP-MS 用于消除光谱干扰。使用硝酸铵和磷酸盐基洗脱液作为 HPLC 分析的流动相，导致总保留时间（6 分钟）缩短，峰分离得到改善。砷形态用 1% HNO 溶液提取，无需净化过程，表现出合理的灵敏度和峰分辨率。通过将该方法应用于 hijiki 海藻认证参考物质（CRM，NMIJ 7405-a）和沉积物及海水的加标空白样品，对优化后的方法进行了验证。所提出的方法测量 CRM 中的 As 浓度为 9.6±0.6μg/kg 干重（dw），接近认证浓度（10.1±0.5μg/kg dw）。六种砷形态在沉积物中的回收率为 87-113%，在海水中的回收率为 99-101%。在实际样品分析中，As 是 hijiki 和 gulfweed 中最丰富的砷形态，而 AsB 是其他海藻物种中的主要砷形态。两种无机砷（As 和 As）和 As 分别是沉积物和海水样品中最主要的砷形态。
