State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.
School of Resource Environment and Earth Science, Yunnan University, Kunming, 650091, People's Republic of China.
Antonie Van Leeuwenhoek. 2019 Dec;112(12):1767-1774. doi: 10.1007/s10482-019-01309-0. Epub 2019 Jul 30.
A Gram-stain negative, aerobic bacterium, designated strain YIM 78456, was isolated from a hot spring sediment, Ngamring county, Tibet, south-west China. The taxonomic position of the isolate was investigated by a polyphasic approach. The novel isolate was found to be aerobic and rod-shaped. Colonies were observed to be pale yellow and circular. The strain was found to grow at pH 7.0-8.0 (optimum, pH 7.0), 45-65 °C (optimum, 55 °C) and in the presence of up to 1.5% NaCl. Comparison of the 16S rRNA gene sequence of strain YIM 78456 and other members of the genus Thermus showed sequence similarities ranging from 90.3 to 97.3%, with strain YIM 78456 showing close sequence similarity to Thermus caliditerrae YIM 77925 (97.3%). The phylogenetic trees based on 16S rRNA gene sequences showed that strain YIM 78456 forms a distinct clade with T. caliditerrae YIM 77925. The predominant menaquinone was identified as MK-8 and the DNA G+C content was determined to be 65.1 mol%. The major cellular fatty acids (> 10%) were identified as iso-C, anteiso-C and iso-C. The polar lipids were found to consist of an aminophospholipid, a phospholipid and glycolipids. On the basis of the morphological and chemotaxonomic characteristics, as well as genotypic data, it is proposed that strain YIM 78456 represents a novel species of the genus Thermus, for which the name Thermus caldilimi sp. nov. is proposed. The type strain is YIM 78456 (= KCTC 52948 = NBRC 113036).
一株革兰氏阴性、需氧细菌，被命名为 YIM 78456 菌株，从中国西南西藏那曲县的一处温泉沉积物中分离得到。通过多相分类法研究了该分离物的分类地位。该新分离株被发现为需氧菌和杆状。菌落呈淡黄色且呈圆形。该菌株被发现可在 pH 值为 7.0-8.0（最佳 pH 值为 7.0）、45-65°C（最佳温度为 55°C）和高达 1.5%NaCl 的环境中生长。与属 Thermus 的其他成员的 16S rRNA 基因序列比较表明，菌株 YIM 78456 与 Thermus caliditerrae YIM 77925 的序列相似性为 90.3-97.3%，与菌株 YIM 78456 与 Thermus caliditerrae YIM 77925 的序列相似性为 97.3%。基于 16S rRNA 基因序列构建的系统发育树表明，菌株 YIM 78456 与 Thermus caliditerrae YIM 77925 形成一个独特的分支。主要的menaquinone 被鉴定为 MK-8，DNA G+C 含量为 65.1 mol%。主要的细胞脂肪酸（>10%）为 iso-C、anteiso-C 和 iso-C。极性脂由一个氨基磷脂、一个磷脂和糖脂组成。基于形态学和化学分类特征以及基因型数据，建议菌株 YIM 78456 代表 Thermus 属的一个新种，命名为 Thermus caldilimi sp. nov.。模式菌株为 YIM 78456（=KCTC 52948=NBRC 113036）。
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