Key Laboratory of Biogeography and Bioresource in Arid Land, CAS, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi, 830011, People's Republic of China.
Antonie Van Leeuwenhoek. 2013 Mar;103(3):513-8. doi: 10.1007/s10482-012-9833-9. Epub 2012 Oct 27.
A Gram-stain negative aerobic bacterium, designated YIM 77924(T), was isolated from a geothermally heated soil sample collected at Rehai National Park, Tengchong, Yunnan province, south-west China. Growth was found to occur from 55 to 75 °C (optimum 65 °C), pH 6.0-8.0 (optimum pH 7.0) and 0-1 % NaCl (w/v). Cells were observed to be rod-shaped and the colonies convex, circular, smooth, yellow and non-transparent. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain YIM 77924(T) belongs to the genus Thermus. The 16S rRNA gene sequence similarity values between strain YIM 77924(T) and other species of the genus Thermus were all below 97 %. The polar lipids of strain YIM 77924(T) were determined to be aminophospholipid, phospholipid and glycolipid. The predominant respiratory quinone was determined to be MK-8 and the G+C content was 66.64 mol%. The major fatty acids identified were iso-C(16:0), iso-C(15:0), iso-C(17:0) and C(16:0). On the basis of the morphological and chemotaxonomic characteristics as well as genotypic data, strain YIM 77924(T) is proposed to represent a novel species, Thermus tengchongensis sp. nov., in the genus Thermus. The type strain is YIM 77924(T) (=KCTC 32025(T) = CCTCC AB2012063(T)).
一株革兰氏阴性需氧细菌，被命名为 YIM 77924(T)，从中国西南部云南省腾冲热海国家公园采集的地热土壤样本中分离得到。该菌的生长条件为 55-75°C（最佳温度 65°C）、pH6.0-8.0（最佳 pH 值为 7.0）和 0-1%NaCl（w/v）。细胞呈杆状，菌落呈凸面、圆形、光滑、黄色、不透明。基于 16S rRNA 基因序列的系统发育分析表明，菌株 YIM 77924(T)属于 Thermus 属。菌株 YIM 77924(T)与其他 Thermus 属种的 16S rRNA 基因序列相似度均低于 97%。菌株 YIM 77924(T)的极性脂包括氨基磷脂、磷脂和糖脂。主要的呼吸醌为 MK-8，G+C 含量为 66.64mol%。鉴定出的主要脂肪酸为 iso-C(16:0)、iso-C(15:0)、iso-C(17:0)和 C(16:0)。根据形态学、化学分类学特征和基因型数据，菌株 YIM 77924(T)代表 Thermus 属中的一个新种，Thermus tengchongensis sp. nov.。模式菌株为 YIM 77924(T)=KCTC 32025(T)=CCTCC AB2012063(T)。
