Pedersen Johannes W, Nøstdal Alexander, Wandall Hans H
Department of Cellular and Molecular Medicine, Copenhagen Center for Glycomics, University of Copenhagen, Copenhagen, Denmark.
Methods Mol Biol. 2019;2024:199-211. doi: 10.1007/978-1-4939-9597-4_12.
Protein microarray is a highly sensitive tool for antibody detection in serum. Monitoring of patients' antibody titers to specific antigens is increasingly employed in the diagnosis of several conditions, ranging from infectious diseases, allergies, autoimmune diseases, and cancer. In this protocol, we present a detailed method for enzymatic generation of disease-specific O-glycopeptides and how to monitor the antibody response to these in serum using microarray technology.
蛋白质微阵列是用于检测血清中抗体的高灵敏度工具。监测患者针对特定抗原的抗体滴度越来越多地应用于多种疾病的诊断，包括传染病、过敏、自身免疫性疾病和癌症。在本方案中，我们介绍了一种酶促生成疾病特异性O-糖肽的详细方法，以及如何使用微阵列技术监测血清中针对这些糖肽的抗体反应。
