Chen Rui, Li Jianjun
Human Health Therapeutics Research Centre, National Research Council Canada, Ottawa, ON, Canada.
Methods Mol Biol. 2019;2024:245-257. doi: 10.1007/978-1-4939-9597-4_15.
Mass spectrometry (MS)-based immunopeptidomics (MHC peptides) is a promising approach for neoantigen discovery through searching MS data against patient-specific protein databases built from exome or transcriptome sequences. MS analysis enables mapping of posttranslational modifications that cannot be predicted from genome sequencing alone but can be more efficient in triggering immune responses. Although MS-based immunopeptidomics has demonstrated its potential in discovering neoantigens as vaccine candidates, the problems such as efficient isolation and sensitive detection (identification) of MHC peptides still hinder the application of its clinical application. Herein, we describe a method based on chemical derivatization and LC-MS/MS for the enhanced detection of MHC peptides.
基于质谱（MS）的免疫肽组学（MHC肽）是一种很有前景的新抗原发现方法，它通过在患者特异性蛋白质数据库中搜索MS数据来实现，该数据库由外显子组或转录组序列构建而成。MS分析能够绘制仅从基因组测序无法预测的翻译后修饰图谱，而这些修饰在触发免疫反应方面可能更有效。尽管基于MS的免疫肽组学已在发现作为疫苗候选物的新抗原方面展现出潜力，但诸如MHC肽的高效分离和灵敏检测（鉴定）等问题仍然阻碍着其临床应用。在此，我们描述了一种基于化学衍生化和液相色谱-串联质谱（LC-MS/MS）的方法，用于增强MHC肽的检测。
