微小 RNA-132 可改善心肌梗死后的心肌重构。

MicroRNA-132 improves myocardial remodeling after myocardial infarction.

机构信息

Department of Cardiology, the People's Hospital of Rizhao, Rizhao, China.

出版信息

Eur Rev Med Pharmacol Sci. 2019 Jul;23(14):6299-6306. doi: 10.26355/eurrev_201907_18452.

DOI:10.26355/eurrev_201907_18452

PMID:31364135

Abstract

OBJECTIVE

To elucidate the potential role of microRNA-132 in myocardial infarction (MI) and its underlying mechanism.

MATERIALS AND METHODS

The myocardial infarction model was established in WT and microRNA-132 KO mice using the LAD ligation method. WT mice were assigned into the control group (LAD ligation for MI) and sham group. After animal procedures, infarct size was calculated using hematoxylin and eosin (HE) staining and cardiac function was evaluated using echocardiography, respectively. By analyzing differentially expressed microRNAs relative to MI in a microarray, microRNA-132 was screened out and further verified by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Hemodynamic parameters and cardiac function indexes in mice were accessed, including scar length/LV length, FS, dp/dtmax, dp/dtmin, ESV, EDV, EF and Tau_w.

RESULTS

QRT-PCR data showed a gradual decrease in microRNA-132 expression in the infarction zone, border zone and remote zone within 7 days after MI. Compared with mice in the control group, microRNA-132 KO mice showed a higher percentage of scar length/LV length at postoperative day 14 and day 28. MicroRNA-132 KO mice showed decreased FS, dp/dtmax and EF, but increased dp/dtmin, ESV and EDV. The injection of different concentrations of microRNA-132 mimics into mice (8 mg/kg, 16 mg/kg and 32 mg/kg) could reduce LVIDD, LVIDs, ESV, EDV, dp/dtmin and Tau_w. However, FS, EF and dp/dtmax increased by the injection of microRNA-132 mimics at postoperative day 28. The injection of 16 mg/kg microRNA-132 mimics significantly reduced the percentage of scar length/LV length in microRNA-132 KO mice than the control group and miR-CO group. After injection of 16 mg/kg microRNA-132 mimics, LVIDD and LVIDs markedly decreased at postoperative day 14 and day 28 compared with the control group and miR-CO group. However, FS was elevated by microRNA-132 mimics.

CONCLUSIONS

MicroRNA-132 is involved in the development of myocardial infarction. The MicroRNA-132 expression is upregulated after myocardial infarction, influencing infarct size and cardiac function.

摘要

目的

阐明 microRNA-132 在心肌梗死（MI）中的潜在作用及其机制。

材料与方法

采用 LAD 结扎法建立 WT 和 microRNA-132 KO 小鼠的心肌梗死模型。WT 小鼠被分为对照组（LAD 结扎致 MI）和假手术组。动物实验完成后，采用苏木精-伊红（HE）染色法计算梗死面积，超声心动图法评估心功能。通过分析微阵列中与 MI 相关的差异表达 microRNA，筛选出 microRNA-132，并通过实时定量聚合酶链反应（qRT-PCR）进一步验证。检测小鼠的血流动力学参数和心功能指标，包括瘢痕长度/LV 长度、FS、dp/dtmax、dp/dtmin、ESV、EDV、EF 和 Tau_w。

结果

qRT-PCR 数据显示，MI 后 7 天内梗死区、交界区和远隔区的 microRNA-132 表达逐渐下降。与对照组相比，microRNA-132 KO 小鼠在术后第 14 天和第 28 天的瘢痕长度/LV 长度百分比更高。microRNA-132 KO 小鼠的 FS、dp/dtmax 和 EF 降低，dp/dtmin、ESV 和 EDV 升高。向小鼠注射不同浓度的 microRNA-132 模拟物（8 mg/kg、16 mg/kg 和 32 mg/kg）可降低 LVIDD、LVIDs、ESV、EDV、dp/dtmin 和 Tau_w。然而，在术后第 28 天，microRNA-132 模拟物的注射可增加 FS、EF 和 dp/dtmax。与对照组和 miR-CO 组相比，向 microRNA-132 KO 小鼠注射 16 mg/kg microRNA-132 模拟物可显著降低瘢痕长度/LV 长度的百分比。术后第 14 天和第 28 天，与对照组和 miR-CO 组相比，注射 16 mg/kg microRNA-132 模拟物后，LVIDD 和 LVIDs 明显降低，而 FS 升高。