Lubec G, Nauer G
University of Vienna, Department of Paediatrics.
Padiatr Padol. 1988;23(2):101-8.
10 hair samples of healthy subjects and 8 hair samples of patients with mucopolysaccharidosis III A were examined by two techniques in the native state and hydrolyzed. For the hydrolyzed samples transmission spectroscopy was used, for the studies on native hair we applied the attenuated reflection technique. The main differences between native and hydrolyzed hair were at 1450, 2337, 2362, 2850, 2870, 2917, 2930, 3080 cm-1. Those wave numbers seem to reflect the supramolecular structure expressed by hydrogen bonding and intramolecular hydrogen bonds. Only the native hair showed those peaks. Other differences, but of quantitative nature only could be found in the infrared spectra. There were no differences between transmission and ATR spectra in patients with mucopolysaccharidose III A and healthy subjects, only the additional peak of the storage material was detectable. In this paper the supramolecular structure of hair on infrared spectroscopy is characterized, giving the basis for futural studies on the alterations in pathology and variations of human hair.
采用两种技术对10份健康受试者的毛发样本和8份黏多糖贮积症ⅢA型患者的毛发样本进行了天然状态及水解状态下的检测。对于水解样本，使用了透射光谱法；对于天然毛发的研究，我们采用了衰减反射技术。天然毛发与水解毛发之间的主要差异出现在1450、2337、2362、2850、2870、2917、2930、3080 cm-1处。这些波数似乎反映了由氢键和分子内氢键所表达的超分子结构。只有天然毛发呈现出这些峰。在红外光谱中还发现了其他差异，但仅是数量上的差异。黏多糖贮积症ⅢA型患者和健康受试者的透射光谱与衰减全反射光谱之间没有差异，仅可检测到储存物质的额外峰。本文对红外光谱下毛发的超分子结构进行了表征，为今后关于人类毛发病理学改变和变异的研究奠定了基础。
