College of Chemistry, Beijing Normal University, Beijing, 100875, China.
Chem Commun (Camb). 2019 Aug 13;55(66):9869-9872. doi: 10.1039/c9cc05008f.
In this work, we designed and synthesized an aggregation-induced emission (AIE)-active tetraphenylethene-decorated pseudo-trisialic acid (TPE3S) and validated its high affinity for Siglecs using microscale thermophoresis techniques. TPE3S was a unique binding-on fluorescent trivalent sialocluster which was successfully utilized for the visualization of Siglecs expressed on the surface of mammalian cells.
在这项工作中，我们设计并合成了一种聚集诱导发光（AIE）活性的四苯乙烯修饰的拟三糖基唾液酸（TPE3S），并利用微量热泳动技术验证了其对 Siglecs 的高亲和力。TPE3S 是一种独特的结合荧光三价唾液酸簇，成功地用于可视化哺乳动物细胞表面表达的 Siglecs。
