Reisfeld Laura, Sacristán Carlos, Sánchez-Sarmiento Angélica María, Costa-Silva Samira, Díaz-Delgado Josué, Groch Kátia Regina, Marigo Juliana, Ewbank Ana Carolina, Favero Cíntia Maria, Guerra Juliana Mariotti, Réssio Rodrigo Albergaria, Cremer Marta Jussara, Esperón Fernando, Catão-Dias José Luiz
Laboratório de Patologia Comparada de Animais Selvagens - LAPCOM, Departamento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brasil.
Aquário de São Paulo, São Paulo, SP, Brasil.
Rev Bras Parasitol Vet. 2019 Jul 29;28(3):499-503. doi: 10.1590/S1984-29612019029.
A juvenile subantarctic fur seal (Arctocephalus tropicalis) found dead in Santa Catarina state, southern Brazil, presented with disseminated verminous pneumonia due to Parafilaroides sp. A concomitant infection with two different gammaherpesviruses was identified by PCR in different tissues; one of them possibly a novel species (tentatively named Otariid herpesvirus 7). Sarcocystis sp. DNA was identified molecularly in skeletal muscle samples with intrasarcoplasmic bradyzoites and no apparent tissue response. All analyzed samples (mandibular, laryngeal, tracheal, and mesenteric lymph nodes, and lung) were PCR-negative for Brucella spp. The most likely cause of death was severe pulmonary parafilaroidiasis. The pathogenic role of the gammaherpesviruses in several of the tissues was not evident. This study describes the pathogenicity of Parafilaroides sp. in a subantarctic fur seal, widens the host range of herpesvirus in pinnipeds, and reports the first molecular identification of Sarcocystis sp. in this species.
在巴西南部圣卡塔琳娜州发现一只死亡的亚南极毛皮海狮幼崽（Arctocephalus tropicalis），其患有由副丝虫属（Parafilaroides sp.）引起的播散性蠕虫性肺炎。通过聚合酶链反应（PCR）在不同组织中鉴定出该海狮同时感染了两种不同的γ疱疹病毒；其中一种可能是新物种（暂命名为海狗疱疹病毒7）。在骨骼肌样本中通过分子方法鉴定出了肉孢子虫属（Sarcocystis sp.）的DNA，肌浆内可见缓殖子，且无明显组织反应。所有分析样本（下颌、喉、气管和肠系膜淋巴结以及肺）布鲁氏菌属PCR检测均为阴性。最可能的死亡原因是严重的肺副丝虫病。γ疱疹病毒在多个组织中的致病作用尚不明显。本研究描述了副丝虫属在亚南极毛皮海狮中的致病性，扩大了疱疹病毒在鳍足类动物中的宿主范围，并首次在该物种中对肉孢子虫属进行了分子鉴定。
