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三种柚子品种采后贮藏过程中抗氧化和细胞壁代谢的变化。

Changes in Metabolisms of Antioxidant and Cell Wall in Three Pummelo Cultivars during Postharvest Storage.

机构信息

Guangdong Key Lab of Sugarcane Improvement & Biorefinery, Guangdong Provincial Bioengineering Institute (Guangzhou Sugarcane Industry Research Institute), Guangdong Academy of Sciences, Guangzhou 510316, China.

Key Laboratory of Plant Resources Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.

出版信息

Biomolecules. 2019 Jul 30;9(8):319. doi: 10.3390/biom9080319.

Abstract

The juice sacs of pummelo fruit is susceptible to softening during storage at 25 °C, which causes quality deterioration and flavor loss during postharvest pummelo storage. This study investigated the changes in metabolisms of antioxidant and cell wall in juice sacs of three pummelo cultivars-Hongroumiyou (HR), Bairoumiyou (BR) and Huangroumiyou (HuR)-during postharvest storage. The results revealed that, with the extension of storage, the juice sacs of three pummelo cultivars exhibited a decrease in total antioxidant capacity (TAC), DPPH and ABTS radical scavenging activity; a decline in total phenols (TP) content and an increase firstly then a decrease in total ascorbic acid (TAA) content; and a decrease in lipoxygenase (LOX) activity and a rise initially, but a decline in activities of ascorbate peroxidase (APX) and glutathione peroxidase (GPX). Additionally, increased water-soluble pectin (WSP), but declined propectin, ionic-soluble pectin (ISP) and chelator-soluble pectin (CSP); as well as an increase from 0 d to 60 d then followed by a decline in activities of pectinesterase (PE), polygalacturonase (PG) and pectate lyase (PL) were observed. These results suggested that the metabolisms of antioxidant and cell wall could result in softening and senescence of pummelo fruit.

摘要

柚子果实的汁囊在 25°C 下贮藏时容易软化,导致柚子果实采后贮藏过程中品质劣变和风味丧失。本研究探讨了三个柚子品种(红肉蜜柚(HR)、白肉蜜柚(BR)和黄肉蜜柚(HuR))汁囊在采后贮藏过程中抗氧化和细胞壁代谢的变化。结果表明,随着贮藏时间的延长,三个柚子品种的汁囊总抗氧化能力(TAC)、DPPH 和 ABTS 自由基清除活性下降;总酚(TP)含量先下降后上升,总抗坏血酸(TAA)含量先上升后下降;脂氧合酶(LOX)活性先升高后降低,过氧化物酶(APX)和谷胱甘肽过氧化物酶(GPX)活性先升高后降低。此外,水溶性果胶(WSP)增加,但原果胶、离子可溶性果胶(ISP)和螯合可溶性果胶(CSP)减少;果胶甲酯酶(PE)、多聚半乳糖醛酸酶(PG)和果胶裂解酶(PL)的活性从 0 天到 60 天增加,然后下降。这些结果表明,抗氧化和细胞壁代谢可能导致柚子果实软化和衰老。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f973/6723824/5c953c36b565/biomolecules-09-00319-g001.jpg

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