Glowska Eliza, Broda Lukasz, Dabert Miroslawa
Adam Mickiewicz University in Poznan, Faculty of Biology, Department of Animal Morphology, Poznan, Poland.
Adam Mickiewicz University in Poznan, Faculty of Biology, Molecular Biology Techniques Laboratory, Poznan, Poland.
Folia Parasitol (Praha). 2019 Jul 23;66:2019.009. doi: 10.14411/fp.2019.009.
Betasyringophiloidus Skoracki, 2011 is a genus of quill mites (Prostigmata: Syringophilidae) that is believed to contain mono-, steno- and polyxenous parasites associated with a wide range of passerine birds (Passeriformes) across the world. In this work we applied the DNA-barcode marker (mitochondrial cytochrome c oxidase subunit I gene fragment, COI) to verify whether Betasyringophiloidus schoeniclus (Skoracki, 2002) and Betasyringophiloidus seiuri (Clark, 1964) are actual steno- and polyxenous species associated with the currently recognised host ranges, or their populations are highly host-specific, cryptic species. Our results revealed that a population living on the Tristram's bunting Emberiza tristrami Swinhoe (Emberizidae) in Russia, so far classified as B. schoeniclus, is a new cryptic species Betasyringophiloidus emberizae sp. nov. Both topologies of the neighbor-joining and maximum likelihood phylogenetic trees as well as genetic distance (11.9% Kimura 2-parameter distance) (K2P) support species status of the mite population from E. tristrami. The same data support previously established conspecific status of B. seiuri found on the ovenbird Seiurus aurocapilla (Linnaeus) (Parulidae) (type host) and the northern waterthrush Parkesia noveboracensis (Gmelin) (Parulidae) and expand its range with a population found on a new host species Icterus pustulatus (Wagler) (Icteridae) with intraspecific K2P distance up to 1.9% and interpopulation distances ranging from 1.3 to 3.1%.
贝氏针尾螨属（Betasyringophiloidus）由斯科拉茨基（Skoracki）于2011年建立，是羽螨的一个属（前气门目：针尾螨科），该属被认为包含与世界各地多种雀形目鸟类（雀形目）相关的单宿主、寡宿主和多宿主寄生虫。在这项研究中，我们应用DNA条形码标记（线粒体细胞色素c氧化酶亚基I基因片段，COI）来验证贝氏黑眉苇莺针尾螨（Betasyringophiloidus schoeniclus）（斯科拉茨基，2002年）和贝氏灶巢鸟针尾螨（Betasyringophiloidus seiuri）（克拉克，1964年）是否为与目前已知宿主范围相关的真正寡宿主和多宿主物种，或者它们的种群是否为高度宿主特异性的隐存物种。我们的结果表明，生活在俄罗斯的灰眉岩鹀（Emberiza tristrami Swinhoe）（鹀科）上的一个种群，目前被归类为贝氏黑眉苇莺针尾螨，是一个新的隐存物种——灰眉岩鹀贝氏针尾螨（Betasyringophiloidus emberizae sp. nov.）。邻接法和最大似然法系统发育树的拓扑结构以及遗传距离（11.9%的Kimura 2-参数距离）（K2P）均支持来自灰眉岩鹀的螨类种群的物种地位。相同的数据支持先前确定的在灶巢鸟（Seiurus aurocapilla）（林奈）（森莺科）（模式宿主）和北水鸫（Parkesia noveboracensis）（格梅林）（森莺科）上发现的贝氏灶巢鸟针尾螨的同种地位，并将其范围扩展到在新宿主物种黄肩黑鹂（Icterus pustulatus）（瓦格勒）（拟鹂科）上发现的一个种群，其种内K2P距离高达1.9%，种群间距离在1.3%至3.1%之间。
