College of Chemistry and Environmental Science, Key Laboratory of Analytical Science and Technology of Hebei Province, Hebei University, 180 Wusi East Road, Baoding, 071002, China.
Mikrochim Acta. 2019 Jul 31;186(8):588. doi: 10.1007/s00604-019-3713-1.
A method is described for non-invasive glucose monitoring of diabetics by means of breath analysis. The metal-organic frameworks (MOFs) ZIF-7, UiO-66 and MOF-5 were chosen as sorbents in packed tubes for sampling and preconcentration of acetone and isopropanol which are established diabetes biomarkers. The MOF UiO-66 was found to be the most appropriate sorbent. Following thermal desorption, acetone and isopropanol where quantified by GC. The method has low limits of detection (0.79-0.84 μg·L) and wide linear ranges (5-2000 μg·L). It is assumed that the good performance of UiO-66 as a sorbent results from its large surface area and unique porous structure, and from van der Waals interactions. The relative standard deviation for six replicate cycles of sampling and preconcentration using one 50 mg UiO-66 packed tube ranged between 2.3 and 6.7% for intra-day assays, and from 2.7 to 4.3% for inter-day assays. A tube packed with 50 mg of UiO-66 packed tube can be used in over 120 cycles of adsorption/desorption without significant loss of collection efficiency. The GC method has been applied for the analysis of diabetic breath samples, and the recoveries from spiked samples ranged from 89.1 to 107.6%. Graphical abstract Schematic presentation of metal-organic frameworks as sorbents combined with thermal desorption-gas chromatography for the determination of acetone and isopropanol in exhaled breath of diabetics.
一种通过呼吸分析对糖尿病患者进行非侵入性血糖监测的方法。选择金属有机骨架（MOF）ZIF-7、UiO-66 和 MOF-5 作为填充管中的吸附剂，用于采样和预浓缩丙酮和异丙醇，这两种物质是已建立的糖尿病生物标志物。研究发现 MOF UiO-66 是最合适的吸附剂。经热解吸后，用 GC 定量分析丙酮和异丙醇。该方法的检测限低（0.79-0.84μg·L），线性范围宽（5-2000μg·L）。人们认为 UiO-66 作为吸附剂的良好性能源于其大的表面积和独特的多孔结构，以及范德华相互作用。使用一根 50mg UiO-66 填充管进行六次重复采样和预浓缩循环的日内测定相对标准偏差在 2.3%至 6.7%之间，日间测定的相对标准偏差在 2.7%至 4.3%之间。一根填充有 50mg UiO-66 的管可在超过 120 个吸附/解吸循环中使用，而不会显著降低收集效率。该 GC 方法已应用于糖尿病患者呼气样本的分析，从加标样品中回收的丙酮和异丙醇的回收率在 89.1%至 107.6%之间。
