Lu Yan, Yin Ying, Zhao Hai-Jun, Wang Yuan, Han Bing-Bing, Wang Shi-Jun
College of Acupuncture-moxibustion and Tuina, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
The First Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011.
Zhen Ci Yan Jiu. 2019 Jun 25;44(6):399-404. doi: 10.13702/j.1000-0607.180836.
To observe the effect of manual acupuncture (MA)＋ electroacupuncture (EA) on changes of neurological function and expression of monocarboxylate transporter 2(MCT2)in cerebral ischemia (CI) rats, so as to explore its mechanism underlying improvement of ischemic cerebrovascular disease.
Eighty male Wistar rats were equally randomized into four groups: normal control (normal), sham operation (sham), model and acupuncture. The CI model was induced by middle cerebral artery occlusion (MCAO) with a thread embolus. Manual acupuncture stimulation (mild twisting reinforcing-reducing method) was applied to "Baihui"(GV20)and "Fengfu"(GV16) for 10 min. EA (1 mA, 2 Hz /15 Hz) was respectively applied to bilateral "Quchi" (LI11) and "Zusanli"(ST36) for 20 min, once per day for 7 days. The neurological deficit severity was evaluated according to Zea Longa's methods. The activity of lactate dehydrogenase (LDH), fructose-6-phosphate kinase (PFK) and pyruvate kinase (PK) in the peri-ischemic cortex tissue was detected by enzymatic chemistry, and the expression of MCT2 detected by immunofluorescence histochemistry, Western blot and quantitative real-time PCR, separately.
After CI and in comparison with the normal and sham groups, the Zea Longa's score, the fluorescence intensity and the expression level of MCT2 protein were significantly increased (<0.01), and the activity of LDH, PFK and PK in the peri-ischemic cortex was significantly decreased in the model group (<0.01). There was no significant change in the relative expression of MCT2 mRNA (>0.05). Following the intervention and in comparison with the model group, the Zea Longa's score was considerably decreased in the acupuncture group (<0.01), the activity of LDH, PFK and PK，and the expression levels of MCT2 protein and mRNA were considerably or further up-regulated in the acupuncture group (<0.01, <0.05).
Acupuncture intervention can improve neurological function in CI rats, which is possibly related with its effects in up-regulating the expression of MCT2 and promoting the utilization of lactate in peri-ischemic cortex.
观察手针（MA）＋电针（EA）对脑缺血（CI）大鼠神经功能变化及单羧酸转运体2（MCT2）表达的影响，以探讨其改善缺血性脑血管病的机制。
将80只雄性Wistar大鼠随机分为四组：正常对照组（正常组）、假手术组（假手术组）、模型组和针刺组。采用线栓法阻断大脑中动脉（MCAO）制备CI模型。对手针刺激“百会”（GV20）和“风府”（GV16）采用捻转补泻手法，针刺10分钟。分别对双侧“曲池”（LI11）和“足三里”（ST36）施加电针（1 mA，2 Hz /15 Hz），持续20分钟，每天1次，共7天。根据Zea Longa法评估神经功能缺损严重程度。采用酶化学法检测缺血周边皮层组织中乳酸脱氢酶（LDH）、磷酸果糖激酶（PFK）和丙酮酸激酶（PK）的活性，分别采用免疫荧光组织化学、蛋白质免疫印迹法和实时定量PCR法检测MCT2的表达。
CI后，与正常组和假手术组相比，模型组Zea Longa评分、MCT2蛋白荧光强度及表达水平显著升高（<0.01），缺血周边皮层LDH、PFK和PK活性显著降低（<0.01）。MCT2 mRNA相对表达量无显著变化（>0.05）。干预后，与模型组相比，针刺组Zea Longa评分显著降低（<0.01），针刺组LDH、PFK和PK活性以及MCT2蛋白和mRNA表达水平显著或进一步上调（<0.01，<0.05）。
针刺干预可改善CI大鼠的神经功能，这可能与其上调MCT2表达及促进缺血周边皮层乳酸利用有关。
