Wang Jin-Hai, Zhang Ting-Zhuo, Li Xing-Lan, Tian Liang, Yuan Bo, Zhao Min, Zhang Zhen-Chang, Du Xiao-Zheng, Jiang Hua
Department of Traditional Chinese Medicine, The Second Hospital of Lanzhou University, Lanzhou 730030, China.
College of Acupuncture-moxibustion and Massage, Gansu University of Traditional Chinese Medicine, Lanzhou 730000.
Zhen Ci Yan Jiu. 2019 Jun 25;44(6):405-11. doi: 10.13702/j.1000-0607.180455.
To observe the influence of scalp acupuncture on cerebral infarct size and expression of IL-10, IL-6, and IL-1β in the para-hippocampal gyrus in acute ischemic cerebrovascular disease(AICD) rats, so as to investigate its mechanisms underlying improvement of AICD.
Forty-eight male SD rats were randomly allocated to normal control (control), AICD model, medication, and scalp acupuncture groups (＝12 per group). The AICD model was established by occlusion of the middle cerebral artery (MCAO). Rats of the medication group received intraperitoneal injection of Ammonium 1-Pyrrolidinedithiocarbamate (APDC, 100 mg•kg•d), once daily for 7 days. Scalp acupuncture stimulation was applied to bilateral "Dingnieqianxiexian" (MS6) once daily for 7 days. Before and after intervention, the neurologic deficit score (NDS) and the neurological score (NS) were evaluated according to Longa's and Schäbitz's methods, respectively. At the end of the intervention, the para-hippocampal gyrus and whole brain were collected respectively. The expression levels of IL-10, IL-6 and IL-1β in the para-hippocampal gyrus tissue were detected by immunohistochemistry, and the cerebral infarct volume of the brain was detected by triphenyltetrazollium chloride (TTC) staining after sectioning.
Following modeling, the NDS, NS and the expression of IL-10, IL-6 and IL-1β in para-hippocampal gyrus were significantly increased in the model group compared with the control group (<0.01). After the intervention, the NDS, NS and infarct volume, and the expression levels of IL-6 and IL-1β in the para-hippocampal gyrus were significantly decreased in both medication and scalp acupuncture groups compared with the model group (<0.05), and the expression of IL-10 was further obviously up-regulated in the scalp acupuncture group (<0.05) rather than in the medication group (>0.05). The effect of scalp acupuncture was obviously superior to that of medication in up-regulating IL-10 expression level (<0.05). No significant differences were found between the medication and scalp acupuncture groups in the levels of NDS, NS, infarct volume, IL-6 and IL-1β proteins (>0.05).
Scalp acupuncture can improve neurological function and reduce infarct volume in AICD rats, which may be associated with its function in up-regulating the expression of IL-10 and in inhibiting the expression of IL-6 and IL-1β to reduce inflammation reaction.
观察头针疗法对急性缺血性脑血管病（AICD）大鼠脑梗死体积及海马旁回白细胞介素-10（IL-10）、白细胞介素-6（IL-6）和白细胞介素-1β（IL-1β）表达的影响，以探讨其改善AICD的作用机制。
将48只雄性SD大鼠随机分为正常对照组（对照组）、AICD模型组、药物组和头针组（每组12只）。采用大脑中动脉闭塞法（MCAO）制备AICD模型。药物组大鼠腹腔注射1-吡咯烷二硫代氨基甲酸盐铵（APDC，100mg•kg•d），每日1次，连续7天。头针组大鼠每日针刺双侧“顶颞前斜线”（MS6），连续7天。干预前后，分别采用Longa法和Schäbitz法评估神经功能缺损评分（NDS）和神经学评分（NS）。干预结束后，分别取海马旁回和全脑。采用免疫组织化学法检测海马旁回组织中IL-10、IL-6和IL-1β的表达水平，切片后采用氯化三苯基四氮唑（TTC）染色检测脑梗死体积。
造模后，模型组NDS、NS及海马旁回IL-10、IL-6和IL-1β表达水平较对照组显著升高（P<0.01）。干预后，药物组和头针组NDS、NS及梗死体积，以及海马旁回IL-6和IL-1β表达水平较模型组显著降低（P<0.05），头针组IL-10表达进一步明显上调（P<0.05），而药物组差异无统计学意义（P>0.05）。头针组上调IL-10表达水平的效果明显优于药物组（P<0.05）。药物组和头针组在NDS、NS、梗死体积、IL-6和IL-1β蛋白水平上差异无统计学意义（P>0.05）。
头针疗法可改善AICD大鼠神经功能，缩小梗死体积，其机制可能与上调IL-10表达、抑制IL-6和IL-1β表达以减轻炎症反应有关。
