Huang Ya-Guang, Tao Wei, Wang Jin-Feng, Cai San-Jin, Yang Song-Bai, Feng Zhi-Tao, Mei Zhi-Gang
College of Medicine, China Three Gorges University, Yichang 443002, Hubei Province, China.
Yichang Hospital of Traditional Chinese Medicine, Clinical Medical College of Traditional Chinese Medicine, China Three Gorges University, Yichang 443003, Hubei Province.
Zhen Ci Yan Jiu. 2019 Jun 25;44(6):459-64. doi: 10.13702/j.1000-0607.180275.
Excessive autophagy is one of the crucial factors of cerebral ischemia-reperfusion injury (CIRI), which has been demonstrated to be one of the targets for acupuncture treatment of ischemic stroke. In the present paper, we make a review about the development of acupuncture intervention induced improvement of CIRI (such as reducing the infarction area, improving learning-memory ability and motor function) by regulating autophagy in animal studies. Outcomes showed that acupuncture intervention can function in 1) inhibiting CIRI-induced increase of the number of lysosomes and autophagic lysosomes, and relieving structural injury of mitochondria, and reducing the number of autophagosome in the central region of the ischemic cerebral cortex tissue; 2) down-regulating the expression of microtubule-associated protein Ⅱ light chain 3 (LC3Ⅱ) and the ratio of LC3-Ⅱ/LC3-Ⅰ in the ischemic cerebral region, and 3) regulating the expression of Beclin 1 (autophagy-related gene), promoting the expression of P62 (autophagy-related adaptor protein). In addition, acupuncture can also regulate phosphoinositide 3 kinase (PI3K)- protein kinase B (AKT)- mammalian target of rapamycin complex 1(mTOR) signaling at different time-points (down-regulation at the early stage and up-regulation at the later stage), and activating AMP-activated protein kinase (AMPK)-mTOR- UNC51-like kinase-1 signaling to relieve cerebral ischemic injury. These results reveal some mechanisms of acupuncture therapy underlying improvement of CIRI and provide experimental basis for clinical application of acupuncture therapy in the treatment of ischemic stroke.
过度自噬是脑缺血再灌注损伤（CIRI）的关键因素之一，已被证明是针刺治疗缺血性中风的靶点之一。在本文中，我们综述了在动物研究中针刺干预通过调节自噬改善CIRI（如缩小梗死面积、改善学习记忆能力和运动功能）的研究进展。结果表明，针刺干预可发挥以下作用：1）抑制CIRI诱导的溶酶体和自噬溶酶体数量增加，减轻线粒体结构损伤，减少缺血性脑皮质组织中心区域自噬体数量；2）下调缺血脑区微管相关蛋白Ⅱ轻链3（LC3Ⅱ）的表达及LC3-Ⅱ/LC3-Ⅰ的比值；3）调节Beclin 1（自噬相关基因）的表达，促进P62（自噬相关衔接蛋白）的表达。此外，针刺还可在不同时间点调节磷脂酰肌醇3激酶（PI3K）-蛋白激酶B（AKT）-雷帕霉素靶蛋白复合物1（mTOR）信号通路（早期下调，后期上调），并激活AMP活化蛋白激酶（AMPK）-mTOR-UNC51样激酶-1信号通路以减轻脑缺血损伤。这些结果揭示了针刺治疗改善CIRI的一些机制，为针刺疗法在缺血性中风治疗中的临床应用提供了实验依据。
