Department of Anatomy, Dr. Arcot Lakshmanasamy Mudaliar Postgraduate Institute of Basic Medical Sciences, University of Madras, Chennai, Tamil Nadu, India.
Department of Electrical and Electronics Engineering, Loyola-ICAM College of Engineering and Technology, Chennai, Tamil Nadu, India.
Aging Male. 2020 Dec;23(5):979-990. doi: 10.1080/13685538.2019.1647160. Epub 2019 Aug 1.
To analyze pathobiology of ischiocavernosus (IC) and bulbospongiosus (BS) muscles in long-term diabetic male rats and its implication on erectile dysfunction (ED).
Male rats were grouped into control and diabetic rats (received single injection of 60 mg/kg bw. of streptozotocin [STZ]). At 120th day, the animals were subjected to various analyses like serum hormone, penile reflex, electromyography of IC and BS muscles, after euthanasia IC and BS muscles were processed for morphological, histology, histometric analysis, immunostaining and immunoblotting synaptophysin, nNOS and NADPH diaphorase histochemistry.
Significant reduction in serum hormone level, penile reflex, reduced action potential or activity in both these muscles and wide range of histological alterations were observed in STZ rats. Muscles showed significant reduction in the diameter, volume and numerical density of the fiber in both muscles of STZ rats. Synaptophysin, nNOS and NADPH diaphorase were significantly reduced in diabetic animal IC and BS.
Severe neuromuscular circuitry alteration in IC and BS. Study concludes that degenerative changes in IC and BS may play a major role in ED in diabetic condition. Indicating diabetic-induced postsynaptic neuronal degeneration along with impaired motor action of the muscle and severe muscle degeneration affecting ED.
分析长期糖尿病雄性大鼠坐骨海绵体（IC）和球海绵体（BS）肌肉的病理生物学变化及其对勃起功能障碍（ED）的影响。
雄性大鼠分为对照组和糖尿病组（给予 60mg/kg bw 链脲佐菌素[STZ]单次注射）。第 120 天，动物进行各种分析，如血清激素、阴茎反射、IC 和 BS 肌肉的肌电图，安乐死后处理 IC 和 BS 肌肉进行形态学、组织学、组织计量学分析、突触素、nNOS 和 NADPH 黄递酶免疫染色和免疫印迹。
STZ 大鼠的血清激素水平、阴茎反射显著降低，这两种肌肉的动作电位或活性降低，并且肌肉组织学发生了广泛的改变。STZ 大鼠的肌肉显示出直径、体积和纤维数量密度的显著降低。IC 和 BS 中的突触素、nNOS 和 NADPH 黄递酶明显减少。
IC 和 BS 中的严重神经肌肉电路改变。研究表明，IC 和 BS 的退行性变化可能在糖尿病状态下的 ED 中起主要作用。这表明糖尿病诱导的突触后神经元变性，以及肌肉运动功能受损和严重的肌肉退化，均会影响 ED。
