Tu Y X, Lebrun E, Bon F, Liu Z J, Van Rapenbusch R
Faculté de Chimie, Université normale du Huazhong, Wuhan, Chine.
C R Acad Sci III. 1988;306(13):385-90.
The trimethoprim-resistant dihydrofolate reductase specified in E. coli by plasmid R 67, when compared, with other enzymes catalyzing the same reaction, to have a dissimilar primary, secondary, tertiary and quaternary structure. In regard to the tertiary structure, we show here that the pteridine binding site, in the plasmid-encoded enzyme, has a geometrical similarity with that of other chromosomal specified reductases.
由质粒R 67在大肠杆菌中所确定的耐甲氧苄啶二氢叶酸还原酶，与催化相同反应的其他酶相比，其一级、二级、三级和四级结构均不相同。关于三级结构，我们在此表明，在质粒编码的酶中，蝶啶结合位点与其他染色体指定的还原酶的蝶啶结合位点在几何结构上具有相似性。
