循环 miR-130 及其靶标 PPAR-γ 可能是 2 型糖尿病合并冠心病患者的潜在生物标志物。
Circulating miR-130 and its target PPAR-γ may be potential biomarkers in patients of coronary artery disease with type 2 diabetes mellitus.
机构信息
Department of Cardiology, Cangzhou Central Hospital, Cangzhou, Hebei, China.
出版信息
Mol Genet Genomic Med. 2019 Sep;7(9):e909. doi: 10.1002/mgg3.909. Epub 2019 Aug 1.
BACKGROUND
Patients of coronary artery disease (CAD) with type 2 diabetes mellitus (DM2) show increased mortality risk than CAD patients without DM2, while few biomarkers can be used to discriminate them.
METHODS
Fifty-nine patients of CAD with DM2 (DM2-CAD group), 79 patients of CAD without DM2 (CAD group), and 63 healthy control subjects were recruited. Circulating miR-130 (miR-130a and miR-130b) and PPAR-γ (peroxisome proliferator-activated receptor gamma) were measured and their Pearson correlation was analyzed. 3' UTR binding prediction and luciferase assay were used to determine the target relationship between miR-130 and PPAR-γ. Receiver operating characteristics (ROC) analysis was performed to test the discrimination ability of miR-130 between DM2-CAD and CAD groups.
RESULTS
miR-130a and miR-130b showed decreased expression in DM2-CAD group when compared with the CAD group and health control. Both bioinformatics and luciferase assays showed that miR-130 could bind the 3' UTR of PPAR-γ. Furthermore, miR-130 negatively correlated with PPAR-γ in both CAD and DM2-CAD group in Pearson's coefficient analysis. Both miR-130a and miR-130b were able to discriminate DM2-CAD group from CAD group and control subjects.
CONCLUSION
Circulating miR-130 may regulate the expression of PPAR-γ and can be used as a biomarker to discriminate DM2-CAD from CAD.
背景
患有 2 型糖尿病（DM2）的冠心病（CAD）患者的死亡率高于无 DM2 的 CAD 患者，但很少有生物标志物可用于区分他们。
方法
招募了 59 例 CAD 合并 DM2 患者（DM2-CAD 组）、79 例 CAD 无 DM2 患者（CAD 组）和 63 例健康对照者。检测循环 miR-130（miR-130a 和 miR-130b）和过氧化物酶体增殖物激活受体γ（peroxisome proliferator-activated receptor gamma，PPAR-γ），并分析其 Pearson 相关性。利用 3'UTR 结合预测和荧光素酶测定来确定 miR-130 与 PPAR-γ 的靶关系。进行受试者工作特征（receiver operating characteristic，ROC）分析以测试 miR-130 在 DM2-CAD 与 CAD 组之间的区分能力。
结果
与 CAD 组和健康对照组相比，DM2-CAD 组 miR-130a 和 miR-130b 的表达降低。生物信息学和荧光素酶测定均显示 miR-130 可与 PPAR-γ 的 3'UTR 结合。此外，Pearson 相关系数分析显示，在 CAD 组和 DM2-CAD 组中，miR-130 与 PPAR-γ 呈负相关。miR-130a 和 miR-130b 均能区分 DM2-CAD 组与 CAD 组和对照组。
结论
循环 miR-130 可能调节 PPAR-γ 的表达，可作为一种生物标志物用于区分 DM2-CAD 与 CAD。
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