Chemistry Department, Yasouj University, Yasouj 75918-74831, Iran.
Chemistry Department, Yasouj University, Yasouj 75918-74831, Iran.
Int J Biol Macromol. 2019 Oct 15;139:40-48. doi: 10.1016/j.ijbiomac.2019.07.196. Epub 2019 Jul 30.
The purpose work is devoted to design of a simple, one-pot and green approach for the synthesis of molecularly imprinted polymer to construct a selective sorbent for pipette-tip solid phase extraction of Rhodamine B from chili powder samples and its subsequence separation and quantification by high performance liquid chromatography-ultraviolet/visible detection. The prepared molecularly imprinted polymer was synthesized using chitosan as versatile natural multi-functional bio-monomer and Rhodamine B as template in aqueous media. The effects of influential parameters (sorbent dosage, flow rate and eluent solvent volume) and their influences on Rhodamine B extraction recovery were examined and optimized by central composite design based response surface methodology as a powerful multivariate optimization tool. Under the optimized conditions, the linear range and limit of detection and quantification of proposed method were achieved to be 0.005-15 mg kg, 0.0015 mg kg and 0.00488 mg kg, respectively, with satisfactory recoveries (>85.0%) and excellent repeatability (relative standard deviation < 6.1%). The easy synthesis conditions as well as satisfactory figures of merit are good indication of applicability of suggested method for extraction and determination of Rhodamine B from chili powder samples in terms of simplicity, cost effectiveness, selectivity and accurate analysis.
这项工作的目的是设计一种简单、一锅煮和绿色的方法来合成分子印迹聚合物，用于构建一种选择性的吸附剂，用于从辣椒粉样品中通过移液器尖端固相萃取罗丹明 B，并通过高效液相色谱-紫外/可见检测对其进行后续分离和定量。所制备的分子印迹聚合物是在水介质中使用壳聚糖作为多功能天然多功能生物单体和罗丹明 B 作为模板合成的。通过基于中心组合设计的响应面法作为一种强大的多元优化工具，考察和优化了影响参数（吸附剂用量、流速和洗脱溶剂体积）及其对罗丹明 B 萃取回收率的影响。在优化条件下，该方法的线性范围、检测限和定量限分别为 0.005-15mgkg、0.0015mgkg 和 0.00488mgkg，回收率>85.0%，重复性好（相对标准偏差<6.1%）。简单、经济有效、选择性好、分析准确，该方法易于合成条件和良好的评价指标表明其适用于从辣椒粉样品中提取和测定罗丹明 B。
