TEDA Institute of Biological Sciences & Biotechnology, Nankai University, TEDA, Tianjin 300457, PR China.
The Key Laboratory of Molecular Microbiology & Technology, Ministry of Education, Tianjin 300071, PR China.
Future Microbiol. 2019 Sep;14:1109-1122. doi: 10.2217/fmb-2019-0118. Epub 2019 Aug 2.
Determination of the virulence regulatory network controlled by the ATP-dependent Lon protease in serovar Typhimurium. The effect of Lon on Typhimurium virulence genes expression was investigated by RNA sequencing, and virulence-associated phenotypes between the wild-type and mutant were compared. , , and flagellar genes were activated, while genes were repressed in the mutant. Accordingly, the mutant exhibited increased adhesion to and invasion of epithelial cells, increased motility and decreased replication in macrophages. The activation of genes by Lon partially accounts for the replication defect of the mutant. A wide range of virulence regulatory functions are governed by Lon in ser. Typhimurium.
赖氨酰（组氨酰）肽酶调控鼠伤寒沙门氏菌毒力的机制研究。 本研究通过 RNA 测序技术分析赖氨酰（组氨酰）肽酶对鼠伤寒沙门氏菌毒力基因表达的影响，并比较野生型和突变型菌株间的表型差异。结果表明，Lon 调控的毒力调控网络中，鞭毛基因和部分毒力相关基因被激活，而部分 SPI-1 基因被抑制。因此，突变型菌株表现出增强的黏附和侵袭上皮细胞的能力、增强的运动性和在巨噬细胞内的复制减少。Lon 对 SPI-1 基因的抑制部分解释了突变型菌株的复制缺陷。 赖氨酰（组氨酰）肽酶在鼠伤寒沙门氏菌中调控多种毒力相关功能。
