Mori M, Iriuchijima T, Kobayashi S
First Department of Internal Medicine, Gunma University, School of Medicine, Maebashi, Japan.
Nihon Naibunpi Gakkai Zasshi. 1988 May 20;64(5):354-61. doi: 10.1507/endocrine1927.64.5_354.
The significant influence of protein synthesis inhibitors on TRH-stimulated secretion of glycosylated TSH was investigated in the rat anterior pituitary in vitro. TRH stimulated secretion of (3H)glucosamine-labeled TSH but did not change incorporation of its labeled precursor into the pituitary TSH. Addition of cycloheximide significantly inhibited both incorporation of (14C)alanine into the pituitary protein and of (3H)glucosamine into the pituitary TSH even 3 hours after incubation, while it did not cause a significant change in secretion of glucosamine-labeled TSH. Apparent inhibition of (14C)alanine incorporation into the pituitary protein was produced by addition of actinomycin D in the 3 and 6 hour-incubation, but the same drug did not cause the significant change in the amount of (3H)-glucosamine-labeled TSH in the anterior pituitary and medium. It is concluded from the present study that TRH plays a potential role in regulating carbohydrate synthesis of TSH prior to secretion, and messenger RNA is not essential for its role in the glycosylation of TSH.
在体外培养的大鼠垂体前叶中，研究了蛋白质合成抑制剂对促甲状腺激素释放激素（TRH）刺激的糖基化促甲状腺激素（TSH）分泌的显著影响。TRH刺激了（3H）葡萄糖胺标记的TSH的分泌，但并未改变其标记前体掺入垂体TSH的情况。即使在孵育3小时后，加入环己酰亚胺也显著抑制了（14C）丙氨酸掺入垂体蛋白以及（3H）葡萄糖胺掺入垂体TSH，然而它并未导致葡萄糖胺标记的TSH分泌出现显著变化。在孵育3小时和6小时时加入放线菌素D可明显抑制（14C）丙氨酸掺入垂体蛋白，但相同药物并未使垂体前叶和培养基中（3H）葡萄糖胺标记的TSH量发生显著变化。从本研究得出结论，TRH在TSH分泌前调节其碳水化合物合成中发挥潜在作用，且信使核糖核酸对其在TSH糖基化中的作用并非必不可少。
