Moses D L, Shille V M
Department of Reproduction, College of Veterinary Medicine, University of Florida, Gainesville 32610.
J Am Vet Med Assoc. 1988 Jun 1;192(11):1541-5.
Twelve anestrous adult Greyhound bitches were used to study a regimen for induction of estrus. Once daily, 7 bitches were given diethylstilbestrol (DES; 5 mg, PO) until sanguineous vaginal discharge and vulvar edema were observed (designated as day 1 of proestrus) and for 2 days thereafter. If no response was elicited after 7 days, a doubled DES dose was given for up to an additional 7 days. Luteinizing hormone (5 mg, IM) was given on day 5 of proestrus, and follicle-stimulating hormone (10 mg, IM) was given on days 9 and 11 of proestrus. Bitches were bred once on day 13. Five bitches were used as a control group; they were given candy tablets for 7 days (first day on tablets, treatment day 1) and 0.9% NaCl (1.0 ml, IM) on treatment days 12, 16, and 18. The 7 bitches treated with DES had a mean proestrus period of 7.7 days and a mean estrus period of 5.7 days up to the day of mating. After mating, they had a mean gestation interval of 64 days and delivered a mean of 4 pups/litter. In 5 bitches, initial treatment with 5 mg of DES/day induced proestrus within 7 days; however, in 2 bitches, additional treatment with 10 mg of DES/day was needed for 5 and 6 days, respectively. Serum estradiol-17 beta and progesterone concentrations remained at base line during the period of DES treatment. Concentrations of both hormones increased after injection with luteinizing hormone and remained high for the next 4 days.(ABSTRACT TRUNCATED AT 250 WORDS)
选用12只处于乏情期的成年灵缇母犬来研究一种诱导发情的方案。7只母犬每天口服5毫克己烯雌酚（DES），直至观察到血性阴道分泌物和外阴水肿（定为发情前期第1天），之后持续2天。如果7天后没有反应，则将DES剂量加倍，最多再持续7天。在发情前期第5天注射5毫克促黄体生成素（IM），在发情前期第9天和第11天注射10毫克促卵泡生成素（IM）。母犬在第13天配种一次。5只母犬作为对照组；它们在7天内每天服用糖片（开始服用糖片的第一天为治疗第1天），并在治疗第12、16和18天注射0.9%氯化钠（1.0毫升，IM）。接受DES治疗的7只母犬，发情前期平均持续7.7天，到配种日发情期平均持续5.7天。配种后，它们的平均妊娠期为64天，平均每窝产4只幼犬。在5只母犬中，每天用5毫克DES进行初始治疗可在7天内诱导发情；然而，在2只母犬中，分别需要额外每天用10毫克DES治疗5天和6天。在DES治疗期间，血清雌二醇-17β和孕酮浓度保持在基线水平。注射促黄体生成素后，两种激素的浓度均升高，并在接下来的4天内保持在高位。（摘要截断于250字）
