Hrdlička Jakub, Gucký Tomáš, Novák Ondřej, Kulkarni Manoj, Gupta Shubhpriya, van Staden Johannes, Doležal Karel
Laboratory of Growth Regulators, Faculty of Science, Institute of Experimental Botany of the Czech Academy of Sciences & Palacký University, Šlechtitelů 27, 78371 Olomouc, Czech Republic.
2Department of Chemical Biology and Genetics, Centre of Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, 783 71 Olomouc, Czech Republic.
Plant Methods. 2019 Jul 25;15:81. doi: 10.1186/s13007-019-0467-z. eCollection 2019.
Karrikins (KARs) are plant growth regulators that promote seed germination and the subsequent growth and development of seedlings of many plant species. In nature they are generated and released by combustion of plant material and promote the restoration of burned ecosystems. Smoke water can be artificially prepared as a saturated extract of all substances in smoke produced by burning plants, and it has various horticultural and agricultural applications.
We have developed, validated and applied the first fast, specific and sensitive method, based on ultra-high performance liquid chromatography-tandem mass spectrometry, for quantifying KARs in smoke water. To assist these efforts and further analyses, standards of the main KARs (which are not commercially available) were synthesized. Due to the complex matrix of smoke waters, two quantification approaches (standard dilution with a structural KAR analogue and standard addition) were compared. The standard addition method allowed absolute quantification of KARs in six of eight smoke water samples of diverse origins and ages.
Our findings reveal differences in both total and relative levels of KARs in smoke water, and indicate that differences in its KAR composition may be linked to variations in its bioactivity.
卡里金（KARs）是一类植物生长调节剂，可促进多种植物种子萌发以及后续幼苗的生长发育。在自然界中，它们由植物材料燃烧产生并释放，促进火烧生态系统的恢复。烟水可通过人工制备，作为燃烧植物产生的烟雾中所有物质的饱和提取物，具有多种园艺和农业应用。
我们开发、验证并应用了基于超高效液相色谱 - 串联质谱的第一种快速、特异且灵敏的方法，用于定量烟水中的卡里金。为辅助这些工作及进一步分析，合成了主要卡里金的标准品（这些标准品无商业出售）。由于烟水基质复杂，比较了两种定量方法（用结构类似的卡里金类似物进行标准稀释和标准加入法）。标准加入法实现了对八个不同来源和年份的烟水样品中的六个样品中卡里金的绝对定量。
我们的研究结果揭示了烟水中卡里金总量和相对水平的差异，并表明其卡里金组成的差异可能与其生物活性的变化有关。
