Zheng Bang-Xiao, Ding Kai
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, PR China.
Faculty of Biological and Environmental Sciences, Ecosystems and Environment Research Programme, University of Helsinki, Niemenkatu 73, Lahti 15140, Finland.
Data Brief. 2019 Mar 12;23:103820. doi: 10.1016/j.dib.2019.103820. eCollection 2019 Apr.
This work included the primer design details of Quantitative Microbial Elemental Cycling (QMEC) method, a high-throughput qPCR method for microbial carbon (C), nitrogen (N), phosphorus (P), sulfur (S) and methane metabolism potential detection and assessment. We designed 36 novel primers based on their amino acid sequences. Via illumina sequencing technology, their phylogenetic taxonomy was identified and analyzed to validate the primer specificity.
这项工作包括定量微生物元素循环（QMEC）方法的引物设计细节，这是一种用于检测和评估微生物碳（C）、氮（N）、磷（P）、硫（S）和甲烷代谢潜力的高通量qPCR方法。我们根据其氨基酸序列设计了36种新型引物。通过Illumina测序技术，对它们的系统发育分类进行了鉴定和分析，以验证引物的特异性。
