评估炎症介质和细胞黏附基因多态性作为猫传染性腹膜炎风险因素的研究。

Evaluation of polymorphisms in inflammatory mediator and cellular adhesion genes as risk factors for feline infectious peritonitis.

作者信息

Kedward-Dixon Helen, Barker Emi N, Tasker Séverine, Kipar Anja, Helps Christopher R

机构信息

The Roundhouse Veterinary Hospital, Glasgow, UK.

Langford Vets, University of Bristol, Langford, UK.

出版信息

J Feline Med Surg. 2020 Jun;22(6):564-570. doi: 10.1177/1098612X19865637. Epub 2019 Aug 2.

DOI:10.1177/1098612X19865637

PMID:31373532

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10814337/

Abstract

OBJECTIVES

Feline infectious peritonitis (FIP) is a high mortality infectious disease. Single nucleotide polymorphisms (SNPs) in the genes encoding interferon gamma (), tumour necrosis factor alpha () and dendritic cell-specific intercellular adhesion molecule-grabbing non-integrin (DC-SIGN; ) have been associated with increased and decreased risk of developing FIP. This study was designed to determine whether these associations were present in a UK population of pedigree cats using samples from cats euthanased with a confirmed diagnosis (FIP, n = 22; non-FIP, n = 10) or clinically healthy cats over 11 years of age (n = 3).

METHODS

DNA was extracted from tissue (n = 32) or blood (n = 3) and PCR performed for regions of and . PCR amplicons were sequenced, each SNP genotype was determined, and genotype/allele frequency for each SNP and FIP status were compared.

RESULTS

No significant association was found between the genotype and FIP status for any SNP analysed. There was a trend for the heterozygous CT genotype at both g.401 and IFNG g.408 to be associated with FIP ( = 0.13), but this genotype was also found in a substantial proportion of non-FIP cats. There was also a trend for the heterozygous CT genotype at g.428 to be associated with FIP ( = 0.06), although most cats with FIP had the CC genotype at this locus. No associations were found between any allele at g.-421, g.1900, g.2276, g.2392 and g.2713 and FIP.

CONCLUSIONS AND RELEVANCE

The use of the and SNPs described to predict the risk of FIP cannot currently be recommended.

摘要

目的

猫传染性腹膜炎（FIP）是一种高致死性传染病。编码γ干扰素（IFNγ）、肿瘤坏死因子α（TNFα）和树突状细胞特异性细胞间黏附分子抓取非整合素（DC-SIGN）的基因中的单核苷酸多态性（SNP）与FIP发病风险的增加和降低有关。本研究旨在利用确诊为FIP后实施安乐死的猫（FIP组，n = 22；非FIP组，n = 10）或11岁以上临床健康猫（n = 3）的样本，确定这些关联在英国纯种猫群体中是否存在。

方法

从组织（n = 32）或血液（n = 3）中提取DNA，并对IFNγ和TNFα区域进行PCR。对PCR扩增产物进行测序，确定每个SNP的基因型，并比较每个SNP的基因型/等位基因频率与FIP状态。

结果

在所分析的任何SNP的基因型与FIP状态之间均未发现显著关联。IFNγ基因g.401和g.408位点的杂合CT基因型有与FIP相关的趋势（P = 0.13），但在相当比例的非FIP猫中也发现了该基因型。TNFα基因g.428位点的杂合CT基因型也有与FIP相关的趋势（P = 0.06），尽管大多数FIP猫在该位点具有CC基因型。在TNFα基因g.-421、IFNγ基因g.1900、DC-SIGN基因g.2276、g.2392和g.2713的任何等位基因与FIP之间均未发现关联。