Key Laboratory of Drug Prevention and Control Technology of Zhejiang Province, Department of Criminal Science and Technology, Zhejiang Police College, Hangzhou, China.
Hangzhou Neoline Technology CO., LTD., Hangzhou, China.
J Clin Lab Anal. 2019 Nov;33(9):e22993. doi: 10.1002/jcla.22993. Epub 2019 Aug 2.
Current chromatographic methods applied for the forensic analysis of methamphetamine are costly, time-consuming, and require complicated pretreatment procedures. Thus, the rapid detection of methamphetamine is a critical and unmet need. In this study, a surface plasmon resonance (SPR) system based on indirect inhibitive immunoassay was designed for the analysis of methamphetamine in forensic oral fluid samples.
For the inhibition immunoassay, the diluted oral fluid was mixed with methamphetamine antibody and then injected into the SPR sensor chip. The biosensor chip was constructed by covalently immobilizing of methamphetamine-bovine serum albumin conjugate onto a carboxymethyl dextran surface at an optimized pH. The concentration of antibody was also optimized.
The SPR biosensor showed good sensitivity with a limit of detection of 0.44 ng/mL and was comparable or lower than the pre-existing methods. The method was finally tested using oral fluid samples from 20 suspected drug abusers in forensic cases, and it provided an acceptable recovery of 113.2%, indicating good anti-interference capability of the SPR sensor.
The SPR biosensor was rapid, reproducible, and had a great potential approach for the forensic detection of methamphetamine.
目前应用于法医学分析甲基苯丙胺的色谱方法成本高、耗时且需要复杂的预处理程序。因此，快速检测甲基苯丙胺是一项关键且未满足的需求。在这项研究中，设计了一种基于间接抑制免疫分析的表面等离子体共振（SPR）系统，用于分析法医口腔液样本中的甲基苯丙胺。
对于抑制免疫分析，将稀释的口腔液与甲基苯丙胺抗体混合，然后注入 SPR 传感器芯片。生物传感器芯片通过将甲基苯丙胺-牛血清白蛋白缀合物在优化 pH 值下共价固定在羧甲基葡聚糖表面上进行构建。还优化了抗体的浓度。
SPR 生物传感器具有良好的灵敏度，检测限为 0.44ng/mL，与现有方法相当或更低。该方法最终使用 20 名法医学疑似药物滥用者的口腔液样本进行了测试，回收率为 113.2%，表明 SPR 传感器具有良好的抗干扰能力。
SPR 生物传感器快速、可重复，是一种用于法医检测甲基苯丙胺的很有前途的方法。
