Tsumura Kampo Research Laboratories, Tsumura & Co., Ibaraki, Japan.
Department of Digestive Surgery and Transplantation, Tokushima University Graduate School of Biomedical Sciences, Tokushima, Japan.
Neurogastroenterol Motil. 2019 Nov;31(11):e13689. doi: 10.1111/nmo.13689. Epub 2019 Aug 2.
The traditional Japanese herbal medicine, daikenchuto (DKT), has been used to treat constipation and postoperative ileus. However, the precise mechanisms involved in the pharmacological effects of DKT remain uncertain. The aim of this study was to clarify the effect of DKT on motor patterns and transit activity in the isolated rat colon.
The entire colon or segments of the proximal colon in rats were isolated and placed in Krebs solution. The motility of the colon was evaluated by analyzing spatiotemporal maps of diameter derived from video imaging and measuring the intraluminal pressure in the anal end of the proximal colon, and the transit time of a plastic bead through the entire isolated colon.
Several types of propagating contractions were observed in the isolated entire colon. When DKT was added to Krebs solution, the frequency of large-extent anal propagating contractions increased. DKT treatment increased the intraluminal pressure in the isolated proximal colon, which was related to the propagating contractions. This effect was abolished by treatment with the neural blocker tetrodotoxin. These findings suggest DKT induced peristaltic contractions in the isolated colon. DKT accelerated colonic transit activity, which was related to peristaltic contractions induction in the colon. These effects were also observed in the colons treated with bethanechol and the active ingredient of DKT, hydroxy-α-sanshool.
Daikenchuto could enhance colonic transit activity by inducing peristaltic contractions, which may be mediated by the activation of the enteric nervous system in the colon.
传统的日本汉方药大建中汤（DKT）已被用于治疗便秘和术后肠麻痹。然而，DKT 药理作用的确切机制仍不清楚。本研究旨在阐明 DKT 对分离大鼠结肠运动模式和转运活动的影响。
将大鼠整个结肠或近端结肠段分离出来，置于 Krebs 溶液中。通过分析视频成像得出的直径时空图谱和测量近端结肠肛门端的腔内压力，以及通过整个分离结肠的塑料珠的转运时间来评估结肠的运动。
在分离的整个结肠中观察到几种类型的传播收缩。当 DKT 添加到 Krebs 溶液中时，大程度肛门传播收缩的频率增加。DKT 处理增加了分离的近端结肠中的腔内压力，这与传播收缩有关。用神经阻滞剂河豚毒素处理可消除这种作用。这些发现表明 DKT 诱导了分离结肠中的蠕动收缩。DKT 加速了结肠转运活动，这与结肠中蠕动收缩的诱导有关。在用氨甲酰胆碱和 DKT 的有效成分羟基-α-山椒素处理的结肠中也观察到了这些作用。
大建中汤可能通过诱导蠕动收缩来增强结肠转运活动，这可能是通过激活结肠中的肠神经系统介导的。
