Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China.
Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China.
Comp Biochem Physiol C Toxicol Pharmacol. 2019 Nov;225:108582. doi: 10.1016/j.cbpc.2019.108582. Epub 2019 Jul 31.
Phenanthrene (PHE) is a common polycyclic aromatic hydrocarbon (PAH) in aquatic environments, and this contaminant can cause adverse effects on teleostean performance. In this study, we exposed the model freshwater fish (zebrafish; Danio rerio) to 300 μg/L PHE for 15 days. Histological analysis demonstrated that liver morphology deteriorated in PHE-exposed zebrafish, and cellular damage in the liver increased. Biological analysis revealed that exposure to PHE elicited significant changes in glutathione S-transferases (GST) and superoxide dismutase (SOD) activities. 476 differentially expressed genes (DEGs) were identified in liver between control and PHE treated groups through the transcriptomic analysis. Gene Ontology enrichment analysis (GO) suggested that PHE exposure induced changes in the expression of genes associated with "lipid transporter activity", "catalytic activity", "metal ion binding", "lipid transport" and "transmembrane transport". Furthermore, the "vitamin digestion and absorption" and "fat digestion and absorption" pathways enriched in Kyoto Encyclopedia of Genes and Genomes analysis (KEGG). Additionally, five candidate biomarkers associated with the PHE response in zebrafish were identified. In conclusion, our results elucidate the physiological and molecular responses to PHE exposure in the liver of zebrafish, and provide a framework for further studies of the mechanisms underlying the toxic effects of polycyclic aromatic hydrocarbons (PAHs) on aquatic organisms.
菲（PHE）是水生环境中常见的多环芳烃（PAH），这种污染物会对硬骨鱼类的性能产生不利影响。在本研究中，我们将模式淡水鱼（斑马鱼；Danio rerio）暴露于 300μg/L 的 PHE 中 15 天。组织学分析表明，暴露于 PHE 中的斑马鱼肝脏形态恶化，肝细胞损伤增加。生物分析表明，暴露于 PHE 会引起谷胱甘肽 S-转移酶（GST）和超氧化物歧化酶（SOD）活性的显著变化。通过转录组分析，在对照组和 PHE 处理组的肝脏中鉴定出 476 个差异表达基因（DEGs）。基因本体论富集分析（GO）表明，PHE 暴露诱导与“脂质转运体活性”、“催化活性”、“金属离子结合”、“脂质转运”和“跨膜转运”相关的基因表达发生变化。此外，京都基因与基因组百科全书分析（KEGG）富集了“维生素消化吸收”和“脂肪消化吸收”途径。此外，还鉴定出与斑马鱼对 PHE 反应相关的五个候选生物标志物。总之，我们的研究结果阐明了 PHE 暴露对斑马鱼肝脏的生理和分子反应，为进一步研究多环芳烃（PAHs）对水生生物毒性作用的机制提供了框架。
