Laboratory of Aquaculture Nutrition and Environmental Health, School of Life Sciences, East China Normal University, Shanghai, 200062, PR China.
Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture, Key Laboratory of Freshwater Aquaculture Genetic and Breeding of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou, 313001, China.
Fish Shellfish Immunol. 2019 Oct;93:463-473. doi: 10.1016/j.fsi.2019.07.082. Epub 2019 Jul 30.
To investigate the effects of arginine (Arg) on the growth, antioxidant capacity, immunity and disease resistance of juvenile Chinese mitten crab, three diets containing Arg levels at 1.72% (control), 2.73% and 3.72% were formulated and fed to Chinese mitten crab (0.22 ± 0.03 g) for eight weeks. The weight gain, ecdysterone and growth hormone in the serum, relative expression of insulin-like growth factor 2 in the hepatopancreas significantly increased in crabs fed the 2.73% and 3.72% Arg diets. The protein and lipid contents significantly increased in crabs fed the 3.72% Arg diet. The feed conversion ratios in crabs fed the diets with Arg additions were lower than in the control. Arg supplementation also enhanced the antioxidative capacity by increasing the activities of superoxide dismutase, catalase and the relative expression of Kelch-like ECH-associated protein 1 gene in the hepatopancreas, which subsequently decreased malondialdehyde content in the hepatopancreas. Besides, Arg also decreased nitric oxide content in the serum and the activity of nitric oxide synthetase in the hepatopancreas. The relative mRNA levels of crustin, relish, lysozyme and cryptocyanin genes were significantly upregulated by Arg supplementation. The activities of acid phosphatase and alkaline phosphatase in the serum significantly increased in crabs fed the 3.72% Arg diet than those in the control. Similarly, the relative mRNA levels of crustin, cryptocyanin and proPO genes were significantly upregulated in crabs fed the 2.73% Arg diet after lipopolysaccharide challenge, and in crabs fed the 3.72% Arg diet after the Poly (I:C) challenge. The crabs fed the 2.73% and 3.72% Arg diets had higher survival rate after bacterial infection than those fed the control diet. This study indicates that the addition of Arg to the diet at 2.7-3.7% can improve the growth, survival, antioxidant capacity, immunity and disease resistance in juvenile Chinese mitten crab.
为了研究精氨酸（Arg）对中华绒螯蟹幼蟹生长、抗氧化能力、免疫力和抗病力的影响，配制了 3 种含 Arg 水平分别为 1.72%（对照）、2.73%和 3.72%的饲料，连续投喂 8 周 0.22±0.03g 的中华绒螯蟹。摄食 2.73%和 3.72%Arg 饲料的中华绒螯蟹体重增重率、血清蜕皮甾酮和生长激素、肝胰腺胰岛素样生长因子 2 相对表达量显著增加。摄食 3.72%Arg 饲料的中华绒螯蟹的蛋白质和脂质含量显著增加。添加 Arg 的饲料组的饲料转化率均低于对照组。Arg 补充还通过增加肝胰腺中超氧化物歧化酶、过氧化氢酶和 Kelch-like ECH-associated protein 1 基因的相对表达来增强抗氧化能力，从而降低肝胰腺中丙二醛的含量。此外，Arg 还降低了血清中一氧化氮的含量和肝胰腺中一氧化氮合酶的活性。Arg 补充可显著上调甲壳质、丰度蛋白、溶菌酶和 cryptocyanin 基因的相对 mRNA 水平。摄食 3.72%Arg 饲料的中华绒螯蟹血清酸性磷酸酶和碱性磷酸酶的活性显著高于对照组。同样，在脂多糖和 Poly（I:C）挑战后，摄食 2.73%Arg 饲料的中华绒螯蟹的甲壳质、 cryptocyanin 和 proPO 基因的相对 mRNA 水平显著上调，而摄食 3.72%Arg 饲料的中华绒螯蟹的 proPO 基因的相对 mRNA 水平显著上调。摄食 2.73%和 3.72%Arg 饲料的中华绒螯蟹的细菌感染后存活率高于摄食对照饲料的中华绒螯蟹。本研究表明，在饲料中添加 2.7-3.7%的 Arg 可以提高中华绒螯蟹幼蟹的生长、存活、抗氧化能力、免疫力和抗病力。
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