Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
Fish Shellfish Immunol. 2020 Sep;104:447-456. doi: 10.1016/j.fsi.2020.06.034. Epub 2020 Jun 14.
T-2 toxin is a secondary metabolite produced by Fusarium spp. that is a major cereal and animal feed contaminant. T-2 toxin has numerous adverse effects on animals, including hepatotoxicity. Arginine (Arg) is closely associated with the regulation of immune responses and antioxidant activity in tissues. The objective of the present study was to evaluate the protective effects of dietary Arg against oxidative damage and immune responses of the hepatopancreas induced by T-2 toxin in Chinese mitten crab. According to the results, 3.17% Arg in the diet decreased alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase activity in the haemolymph significantly, when compared with the levels of activity in the T-2 toxin group. Arg supplementation also increased superoxide dismutase and glutathione peroxidase activity, while decreasing malondialdehyde concentrations in the hepatopancreas, when compared with the levels in the T-2 toxin group. In addition, 3.17% Arg in the diet increased acid phosphatase and alkaline phosphatase activity in the hepatopancreas, as well as albumin concentrations in the haemolymph, when compared with the T-2 toxin group. Dietary Arg also regulated the expression of antioxidant enzyme-related genes (mitochondrial manganese superoxide dismutase, cytosolic manganese superoxide dismutase, and catalase) and immune related genes (prophenoloxidase, NF-κB-like transcription factor Relish, and lipopolysaccharide-induced TNF-α factor) to alleviate the damage associated with the T-2 toxin. Furthermore, Arg ameliorated damage to the hepatopancreas microstructure in the crabs. The results of the present study indicate that dietary Arg could enhance the antioxidant and immune capacity of Chinese mitten crab against oxidative damage and immune injury to the hepatopancreas induced by T-2 toxin.
T-2 毒素是镰刀菌属产生的一种次生代谢物，是主要的谷物和动物饲料污染物。T-2 毒素对动物有许多不良影响，包括肝毒性。精氨酸（Arg）与组织中免疫反应和抗氧化活性的调节密切相关。本研究旨在评估日粮 Arg 对中华绒螯蟹 T-2 毒素诱导的肝胰腺氧化损伤和免疫反应的保护作用。结果表明，日粮中 3.17%的 Arg 可显著降低血淋巴中丙氨酸氨基转移酶、天冬氨酸氨基转移酶和碱性磷酸酶的活性，与 T-2 毒素组相比。Arg 补充还可增加超氧化物歧化酶和谷胱甘肽过氧化物酶的活性，同时降低肝胰腺中的丙二醛浓度，与 T-2 毒素组相比。此外，日粮中 3.17%的 Arg 可增加肝胰腺中的酸性磷酸酶和碱性磷酸酶活性以及血淋巴中的白蛋白浓度，与 T-2 毒素组相比。日粮 Arg 还可调节抗氧化酶相关基因（线粒体锰超氧化物歧化酶、细胞质锰超氧化物歧化酶和过氧化氢酶）和免疫相关基因（酚氧化酶原、NF-κB 样转录因子 Relish 和脂多糖诱导的 TNF-α 因子）的表达，以减轻与 T-2 毒素相关的损伤。此外，Arg 改善了螃蟹肝胰腺的微观结构损伤。本研究结果表明，日粮 Arg 可增强中华绒螯蟹的抗氧化和免疫能力，抵御 T-2 毒素对肝胰腺的氧化损伤和免疫损伤。
