Chemical Engineering Laboratory, Faculty of Sciences and Centre for Advanced Scientific Research (CICA), University of Coruña, Rua da Fraga 10, E - 15008 A Coruña, Spain.
Department of Biology, University of Padova, Via U. Bassi 58/b, Padova 35131, Italy; CRIBI Biotechnology Center, University of Padova, Viale G. Colombo 3, Padova 35131, Italy.
Bioresour Technol. 2019 Nov;291:121817. doi: 10.1016/j.biortech.2019.121817. Epub 2019 Jul 16.
This work explored the production of volatile fatty acids (VFA) through the anaerobic digestion of sewage sludge (SS). The first experiment took place at batch scale to evaluate the combined effect of using a thermal pre-treatment (120 °C, 15 min) and different Substrate/Inoculum ratios (S/I) (1, 2, 4 and 6 g VS substrate/g VS inoculum) on the acidogenic potential of the SS. The results showed that the thermal pre-treatment influenced positively the degree of acidification of the SS at low S/I ratios, reaching maximum of 45%. Afterwards, a continuous lab-scale experiment, was set-up to study two ranges of organic loading rates (OLR): 1300-1600 mg COD L d and 2400-3500 mg COD L d. The highest degree of acidification (22%) was achieved at the lowest OLR. Analysis of the microbial community in the reactor revealed that OTUs most abundant present genes related with amino acids and carbohydrates fermentation being crucial for VFA production.
本研究探讨了通过厌氧消化污水污泥（SS）生产挥发性脂肪酸（VFA）的过程。第一个实验在批量规模下进行，以评估使用热预处理（120°C，15 分钟）和不同底物/接种物比（S/I）（1、2、4 和 6 g VS 底物/g VS 接种物）对 SS 的产酸潜力的综合影响。结果表明，热预处理对低 S/I 比下 SS 的酸化程度有积极影响，达到最高 45%。随后，建立了一个连续的实验室规模实验，研究两个有机负荷率（OLR）范围：1300-1600 mg COD L d 和 2400-3500 mg COD L d。在最低 OLR 下实现了最高的酸化程度（22%）。对反应器中微生物群落的分析表明，丰度最高的 OTUs 存在与氨基酸和碳水化合物发酵相关的基因，这对于 VFA 的生产至关重要。
