Alzoman Nourah Z, Maher Hadir M, Shehata Shereen M, Abanmy Norah O
College of Pharmacy, Department of Pharmaceutical Chemistry, King Saud University, Riyadh, Saudi Arabia.
Faculty of Pharmacy, Department of Pharmaceutical Analytical Chemistry, University of Alexandria, Alexandria, Egypt.
Biomed Chromatogr. 2019 Dec;33(12):e4674. doi: 10.1002/bmc.4674. Epub 2019 Sep 1.
Tyrosine kinase inhibitor treatments for chronic myeloid leukaemia based on nilotinib (NIL), dasatinib (DAS) and imatinib (IMA) have improved patient quality of life and have turned chronic myeloid leukemia from a fatal disease into a chronic disease. Dandelion is a rich source of phenolic compounds with strong biological properties, and the effects of using this plant in the treatment of different illnesses can be linked to the presence of various polyphenols found in the different parts of the plant. Thus, dandelion can potentially be used as a nutraceutical (dietary antioxidant) to prevent different disorders associated with oxidative stress, i.e. cardiovascular disorders, cancer and inflammatory processes. Mutual interference between a drug and a food constituent may result in altered pharmacokinetics of the drug and undesired or even dangerous clinical situations. In the present study, a bioanalytical ultra performance liquid chromatography-tandem mass spectrometer (UPLC-MS/MS) method was developed and validated for the quantification of DAS, IMA and NIL in rat plasma. Sample preparation was carried out using solid-phase extraction with C cartridges with a good extraction recovery of ≥94.37% for the three drugs. The method was fully validated as per the US Food and Drug Administration guidelines.
基于尼罗替尼（NIL）、达沙替尼（DAS）和伊马替尼（IMA）的酪氨酸激酶抑制剂疗法改善了慢性髓性白血病患者的生活质量，并使慢性髓性白血病从一种致命疾病转变为一种慢性疾病。蒲公英富含具有强大生物学特性的酚类化合物，使用这种植物治疗不同疾病的效果可能与该植物不同部位发现的各种多酚的存在有关。因此，蒲公英有可能用作营养保健品（膳食抗氧化剂），以预防与氧化应激相关的不同疾病，即心血管疾病、癌症和炎症过程。药物与食物成分之间的相互干扰可能会导致药物的药代动力学改变以及出现不良甚至危险的临床情况。在本研究中，开发并验证了一种生物分析超高效液相色谱-串联质谱仪（UPLC-MS/MS）方法，用于定量大鼠血浆中的DAS、IMA和NIL。使用C柱进行固相萃取制备样品，三种药物的萃取回收率良好，均≥94.37%。该方法已按照美国食品药品监督管理局的指南进行了全面验证。
