Key Laboratory of Advanced Drug Preparation Technologies, Ministry of Education of China, o-innovation Center of Henan Province for New Drug R & D and Preclinical Safety, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, Henan, 450001, PR China.
Key Laboratory of Advanced Drug Preparation Technologies, Ministry of Education of China, o-innovation Center of Henan Province for New Drug R & D and Preclinical Safety, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, Henan, 450001, PR China.
Eur J Pharmacol. 2019 Oct 15;861:172587. doi: 10.1016/j.ejphar.2019.172587. Epub 2019 Aug 1.
Oridonin is a diterpenoid isolated from Rabdosia rubescens (Hemsl.) Hara, a well-known herbal tea in China with many health benefits. To provide a better understanding of the potential cardioprotective effect of Oridonin, we investigated the metabolic alterations in heart tissue and serum of rat subjected to myocardial ischemia/reperfusion (MI/R) injury with or without pretreatment of Oridonin by UPLC-MS/MS metabolomics approach. Rats were randomly divided into groups as follows: Control, Sham, MI/R and pretreated with Oridonin (10 mg/kg)+MI/R. After 24 h of reperfusion, heart tissue and serum were collected for biochemical and metabolomic analysis. Pretreatment with Oridonin significantly decreased infarct size and reversed the abnormal elevated myocardial zymogram in serum. Moreover, Oridonin regulated several metabolic pathways, including glycolysis, branched chain amino acid, kynurenine, arginine, glutamine and bile acid metabolism. Our results suggest that Oridonin indeed displays outstanding cardioprotective effect mainly by regulating energy and amino acid metabolism.
冬凌草甲素是从中国一种著名的草药茶——冬凌草（Rabdosia rubescens（Hemsl.）Hara）中分离得到的二萜类化合物，具有多种健康益处。为了更好地了解冬凌草甲素的潜在心脏保护作用，我们采用 UPLC-MS/MS 代谢组学方法研究了心肌缺血/再灌注（MI/R）损伤大鼠心脏组织和血清中的代谢变化，以及冬凌草甲素预处理对其的影响。大鼠随机分为以下几组：对照组、假手术组、MI/R 组和冬凌草甲素预处理+MI/R 组。再灌注 24 小时后，采集心脏组织和血清进行生化和代谢组学分析。冬凌草甲素预处理显著减少了梗死面积，并逆转了血清中异常升高的心肌酶谱。此外，冬凌草甲素调节了几种代谢途径，包括糖酵解、支链氨基酸、犬尿氨酸、精氨酸、谷氨酰胺和胆汁酸代谢。我们的结果表明，冬凌草甲素确实通过调节能量和氨基酸代谢发挥出色的心脏保护作用。
