淋巴瘤相关巨噬细胞与霍奇金-里德-斯特恩伯格细胞比值是经典霍奇金淋巴瘤患者的不良预后因素。

The Lymphoma-Associated Macrophage to Hodgkin-Reed-Sternberg Cell Ratio Is a Poor Prognostic Factor in Classic Hodgkin Lymphoma Patients.

机构信息

Department of Hemato-Oncology, Faculty of Medicine and Dentistry, Palacký University and University Hospital, Olomouc, Czech Republic.

出版信息

Clin Lymphoma Myeloma Leuk. 2019 Oct;19(10):e573-e580. doi: 10.1016/j.clml.2019.07.001. Epub 2019 Jul 5.

DOI:10.1016/j.clml.2019.07.001

PMID:31377208

Abstract

BACKGROUND

Despite the relatively high rate of curability, approximately 20% to 30% of patients with classic Hodgkin lymphoma relapse. Hodgkin-Reed-Sternberg (HRS) cells:lymphoma-associated macrophages (LAMs) cross talk promotes tumor growth and resistance to therapy. The aim of the study was to assess the prognostic role of the LAM to HRS ratio (LHR) in lymph node biopsies using a novel automated system for scanning large sample areas.

PATIENTS AND METHODS

High-quality tissue samples obtained from 71 patients and stained with anti-CD30 and anti-CD68 were analyzed using the TissueFAXS (TissueGnostics).

RESULTS

A high LHR was associated with inferior 5-year progression-free survival (PFS; 50.0% vs. 79.3%; P = .032) and overall survival (OS; 65.4% vs. 92.3%; P = .012). Multivariate Cox regression identified the high LHR as an unfavorable prognostic factor for PFS (hazard ratio [HR], 3.07; P = .029) and OS (HR, 4.56; P = .025).

CONCLUSION

A high LHR at diagnosis is associated with a higher risk of lymphoma progression or death. Automated image analysis is a new tool that can overcome technical limitations of by microarray samples in lymphomas with high intratumor heterogeneity.

摘要

背景

尽管经典霍奇金淋巴瘤的治愈率相对较高，但仍有约 20%至 30%的患者会复发。霍奇金-里德-斯特恩伯格（HRS）细胞与淋巴瘤相关巨噬细胞（LAMs）的相互作用促进了肿瘤的生长和对治疗的耐药性。本研究旨在使用新型自动扫描系统评估淋巴结活检中 LAM 与 HRS 细胞比值（LHR）的预后作用。

患者与方法

使用 TissueGnostics 公司的 TissueFAXS 对 71 名患者的高质量组织样本进行了抗 CD30 和抗 CD68 染色分析。

结果

高 LHR 与 5 年无进展生存率（PFS；50.0% vs. 79.3%；P =.032）和总生存率（OS；65.4% vs. 92.3%；P =.012）较差相关。多因素 Cox 回归分析确定高 LHR 是 PFS（风险比 [HR]，3.07；P =.029）和 OS（HR，4.56；P =.025）的不良预后因素。