CoNISMa, Piazzale Flaminio 9, 00196, Roma, Italy.
Department of Biology, Environmental Sciences and Technologies, University of Salento, 73100, Lecce, Italy.
Environ Pollut. 2019 Nov;254(Pt A):112977. doi: 10.1016/j.envpol.2019.112977. Epub 2019 Jul 27.
Glyphosate, as a broad-spectrum herbicide, is frequently detected in water and several studies have investigated its effects on several freshwater aquatic organisms. Yet, only few investigations have been performed on marine macroalgae. Here, we studied both the metabolomics responses and the effect on primary production in the endemic brown algae Fucus virsoides exposed to different concentration (0, 0.5, 1.5 and 2.5 mg L) of a commercial glyphosate-based herbicide, namely Roundup®. Our results show that Roundup® significantly reduced quantum yield of photosynthesis (F/F) and caused alteration in the metabolomic profiles of exposed thalli compared to controls. Together with the decrease in the aromatic amino acids (phenylalanine and tyrosine), an increase in shikimate content was detected. The branched-amino acids differently varied according to levels of herbicide exposure, as well as observed for the content of choline, formate, glucose, malonate and fumarate. Our results suggest that marine primary producers could be largely affected by the agricultural land use, this asking for further studies addressing the ecosystem-level effects of glyphosate-based herbicides in coastal waters.
草甘膦作为一种广谱除草剂，经常在水中被检测到，已有多项研究调查了它对几种淡水水生生物的影响。然而，对海洋大型藻类的研究却很少。在这里，我们研究了暴露于不同浓度（0、0.5、1.5 和 2.5 mg/L）的商业草甘膦除草剂 Roundup®的内地产棕色海藻石莼的代谢组学反应和对初级生产力的影响。我们的结果表明，与对照组相比，Roundup®显著降低了光合作用的量子产率（F/F），并导致暴露组织的代谢组学图谱发生改变。与芳香族氨基酸（苯丙氨酸和酪氨酸）的减少一起，检测到莽草酸含量增加。支链氨基酸的变化根据除草剂暴露水平而不同，同样观察到胆碱、甲酸盐、葡萄糖、丙二酸盐和富马酸盐的含量变化。我们的结果表明，海洋初级生产者可能会受到农业土地利用的极大影响，这需要进一步的研究来解决沿海水域中草甘膦类除草剂对生态系统水平的影响。
