Kimura H, Yamaguchi Y, Obata S, Yusa T, Sekine Y
Dept. of Surgery, School of Medicine, Chiba University.
Gan To Kagaku Ryoho. 1988 Aug;15(8 Pt 2):2401-4.
Bronchial arterial infusion (BAI) of Lymphokine activated killer (LAK) cells, stimulated in vitro by autologous tumor cells, was performed for a primary lung cancer patient, a 47-year-old male patient with primary squamous cell carcinoma of the lung who underwent probe thoracotomy and had part of the tumor tissue removed. Peripheral blood lymphocytes were cultured in 500 U/ml of recombinant IL-2(Shionogi Pharm. S6820) for 9 days after in vitro stimulation with mitomycin C-treated primary tumor cells for 3 days. These cells (designated St-LAK cells) were inoculated from the bronchial artery of patients who received 60 Gy irradiation of the primary site and mediastinal lymph nodes. The tumor regressed significantly from 8 X 7 to 3 X 2.5 cm in diameter. The advantages of LAK-BAI using St-LAK cells with irradiation were discussed.
对一名原发性肺癌患者进行了支气管动脉灌注（BAI），该患者为47岁男性，患有原发性肺鳞状细胞癌，接受了胸腔镜探查并切除了部分肿瘤组织。外周血淋巴细胞在体外经丝裂霉素C处理的原发性肿瘤细胞刺激3天后，于500 U/ml的重组白细胞介素-2（盐野义制药S6820）中培养9天。这些细胞（称为St-LAK细胞）从接受了原发部位和纵隔淋巴结60 Gy照射的患者支气管动脉注入。肿瘤直径从8×7厘米显著缩小至3×2.5厘米。讨论了使用经照射的St-LAK细胞进行LAK-BAI的优势。
