Cooper E S, Polesky H F, Harrison C R, Schmidt P J, Simon T L
Blood Bank, Alton Ochsner Medical Foundation, New Orleans, LA 70121.
Arch Pathol Lab Med. 1988 Sep;112(9):878-82.
In 1986, a total of 2781 laboratories subscribed to the Comprehensive Blood Bank Survey of the College of American Pathologists (CAP) (Skokie, Ill). Performance of these laboratories on the ABO and Rh challenges was good, with 99.7% to 99.9% of the participants reporting the correct response. Antibody identification showed less consistency (45.4% to 97.5%). These challenges consisted of two serum samples with multiple antibodies, one serum sample with antibody to a high-incidence antigen, and one serum sample with anti-M. Performance on the cross match ranged from 95.0% to 99.8% correct, with one exception. This exception was an intended incompatible cross match with the serum containing anti-M, which was reported as incompatible by 56.2% of participants. The antigen-typing challenges ranged from 98.3% to 100% correct, but three typings proved to be a problem. Two samples, when typed for the c antigen, were correctly typed 70.4% of the time and 50.1% of the time. One of these samples, when typed for the E antigen, was correctly typed only 30% of the time. This discrepancy proved to be the result of inadvertent pooling of units of different phenotypes in the sample preparation process.
1986年,共有2781家实验室参与了美国病理学家学会(CAP,伊利诺伊州斯科基)的综合血库调查。这些实验室在ABO和Rh血型鉴定方面表现良好,99.7%至99.9%的参与者报告了正确结果。抗体鉴定的一致性较低(45.4%至97.5%)。这些鉴定包括两份含有多种抗体的血清样本、一份含有针对高频率抗原的抗体的血清样本以及一份含有抗-M抗体的血清样本。交叉配血的正确率在95.0%至99.8%之间,但有一个例外。这个例外是一份与含有抗-M抗体的血清进行的预期不配合交叉配血,56.2%的参与者报告其为不配合。抗原分型的正确率在98.3%至100%之间,但有三种分型出现了问题。两份样本在进行c抗原分型时,正确分型的比例分别为70.4%和50.1%。其中一份样本在进行E抗原分型时,正确分型的比例仅为30%。事实证明,这种差异是样本制备过程中不同表型的单位意外混合的结果。