Department of Medical Oncology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China.
Department of Otorhinolaryngology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.
J Zhejiang Univ Sci B. 2019;20(9):776-780. doi: 10.1631/jzus.B1900179.
Laryngeal squamous cell carcinoma (LSCC) is the most common type of head and neck squamous cell carcinoma (HNSCC) worldwide. Protein phosphatase 2A (PP2A) dysfunction has been widely reported in a broad range of malignancies due to its distinctive role in miscellaneous cellular processes. However, it is poorly understood whether aberrant alterations of PP2A are involved in the network of oncogenic events in LSCC. Here, we detected a panel of PP2A-associated proteins using western blot in both laryngeal squamous cell carcinoma tissues and paired adjacent normal tissues from patients (Data S1). We found that phospho-PP2A/C (Y307), α4, cancerous inhibitor of protein phosphatase 2A (CIP2A), Akt, ezrin, phospho-ezrin (T567), 14-3-3, and focal adhesion kinase (FAK) showed increased expression levels in carcinoma tissues relative to normal tissues, while phospho-Akt (T308) showed decreased levels. Our study, thus, provides a rationale for targeting PP2A to develop novel therapies and proposes a combination of interrelated biomarkers for the diagnostic evaluation and prognosis prediction in LSCC.
喉鳞状细胞癌（LSCC）是全球最常见的头颈部鳞状细胞癌（HNSCC）类型。由于其在各种细胞过程中独特的作用，蛋白磷酸酶 2A（PP2A）功能障碍已在广泛的恶性肿瘤中得到广泛报道。然而，目前尚不清楚 PP2A 的异常改变是否参与 LSCC 致癌事件网络。在这里，我们使用 Western blot 在患者的喉鳞状细胞癌组织和配对的相邻正常组织中检测了一组 PP2A 相关蛋白（数据 S1）。我们发现磷酸化-PP2A/C（Y307）、α4、癌性蛋白磷酸酶 2A 抑制剂（CIP2A）、Akt、ezrin、磷酸化 ezrin（T567）、14-3-3 和黏着斑激酶（FAK）在癌组织中的表达水平相对正常组织升高，而磷酸化-Akt（T308）的表达水平降低。因此，我们的研究为靶向 PP2A 开发新型治疗方法提供了依据，并提出了相关生物标志物的组合，用于 LSCC 的诊断评估和预后预测。
