Remy L, Reynier M O, Hashieh I A, Gerolami A
I.N.S.E.R.M. Faculté de Médecine Timone, Marseille.
C R Acad Sci III. 1988;307(2):63-8.
A few hours after plating, isolated rat hepatocytes reassociate into clusters and differentiate intercellular cavities bordered by junctional complexes. These structures show a great resemblance to bile canaliculi seen in vivo. Intracellular lumens surrounded by microvilli are observed in the cytoplasm of some cultured hepatocytes. The formation of these structures, which contain an osmiophilic substance, probably results from modifications in the functioning of the secretory apparatus. It can be speculated that these intracellular lumens may contribute to the formation of new canaliculi differentiated between reassociated cells.
接种后数小时，分离的大鼠肝细胞重新聚集成团，并分化出由连接复合体界定的细胞间腔隙。这些结构与体内所见的胆小管极为相似。在一些培养的肝细胞的细胞质中观察到由微绒毛包围的细胞内管腔。这些含有嗜锇物质的结构的形成可能是由于分泌装置功能的改变所致。可以推测，这些细胞内管腔可能有助于重新聚集的细胞之间分化出新的胆小管。
